鲤NADH泛醌氧化还原酶亚基3基因的克隆及低温适应相关性分析

根据鲤低温下特异表达的基因（片段）序列设计引物，采用2轮菌落PCR方法对鲤脑全长cDNA文库中的克隆进行筛选，确定了基因序列152在文库中的位置，经生物信息学比对分析确定其为NADH泛醌氧化还原酶亚基3基因.这一基因在低温下的特异表达，对于低温下鱼类机体的供能，起着重要作用.结果还表明，NADH泛醌氧化还原酶亚基3基因在鲤氧化呼吸链电子传递系统中与低温适应性状在一定程度上存在相关性.

Cloning and Correlation Analysis of Cold Adaptation of NADH-Quinone Oxidoreductase 3 Subunit Gene from Common Carp(Cyprinus Carpio)

Genes expressed specifically at low temperature were used to design primers and to screen brain full-length cDNA library of common carp (Cyprinus carpio) through two rounds of clonal PCR method.After that,gene 152 was discovered and sequenced,and determined finally as NADH-quinone oxidoreductase 3 subunit gene using homologous searches.This gene was confirmed to play an important role as the force generators for fish in a low water environment.In addition,NADH-quinone oxidoreductase 3 subunit gene was associated with cold adaptation of common carp to some extent in oxidation of respiratory chain electron transport system.