Presence of specific binding sites for [3H]sarcophytol-A in cultured human skin fibroblasts |
| |
Authors: | T Nakagane R Ishitani |
| |
Institution: | (1) Group of Neuropharmacology, Josai University, 350-02 Sakado, Saitama, (Japan) |
| |
Abstract: | Summary The presence of specific binding sites for 3H]sarcophytol-A in human skin fibroblasts was examined using biochemical and morphological methods. The displacement studies clearly revealed that high (KD=31.0 nM) and low (KD=6.05 M) affinity sites were present in the intact cells. Moreover, autoradiographic studies using light microscopy revealed that the specific binding sites may exist in boththe cytoplasm and the nuclei. |
| |
Keywords: | Sarcophytol-A anti-promoter specific binding sites human skin fibroblasts autoradiography |
本文献已被 SpringerLink 等数据库收录! |