| 应用毛细管电泳表征噬菌体与丹酚酸B的相互作用 |
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| 引用本文： | 刘书朋,孙烨,郭薇,徐宇虹.应用毛细管电泳表征噬菌体与丹酚酸B的相互作用[J].上海大学学报(自然科学版),2008,14(1):96-99. |
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| 作者姓名： | 刘书朋 孙烨 郭薇 徐宇虹 |
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| 作者单位： | 1.上海交通大学 生命科学技术学院，上海 200240；; 2.上海交通大学 药学院，上海 200240；;3.上海大学 通信与信息工程学院，上海 200072) |
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| 摘 要： | 基于Hummel-Dreyer毛细管电泳方法研究经噬菌体展示库生物淘洗亲和筛选出的噬菌体和丹酚酸B之间的结合作用，结合系数由PRISM软件中Scatchard方程曲线拟合计算得出.实验结果表明，筛选出的该噬菌体克隆的表面蛋白和丹酚酸B具有特异性的结合作用，也说明利用毛细管电泳测定噬菌体和丹酚酸B的结合系数是可行的.
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| 关 键 词： | 丹酚酸B 毛细管电泳 噬菌体 |
| 文章编号： | 1007-2861（2008）01-0096-04 |
| 收稿时间： | 2006-11-24 |
| 修稿时间： | 2006年11月24 |
Characterization of Interaction between Salvianolic Acid Band Phages Using Capillary Electrophoresis |
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| LIU Shu-peng,SUN Ye,GUO Wei,XU Yu-hong.Characterization of Interaction between Salvianolic Acid Band Phages Using Capillary Electrophoresis[J].Journal of Shanghai University(Natural Science),2008,14(1):96-99. |
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| Authors: | LIU Shu-peng SUN Ye GUO Wei XU Yu-hong |
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| Institution: | 1. College of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China; ;2. School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China; ;3. School of Communication and Information Engineering, Shanghai University, Shanghai 200072, China |
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| Abstract: | Binding activity between Salvianolic acid B and selected phages from affinity biopanning in phage display library were studied with capillary electrophoresis based on the Hummel-Dreyer methods. The binding constant was calculated with Scatchard analysis using PRISM software. The results indicate that the surface proteins expressed by the phage clone interact specifically with Salvianolie acid B, and it is feasible to determine the binding constant between the selected phage and Salvianolic acid B using CE. |
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| Keywords: | Salvianolic acid B phage capillary electrophoresis |
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