| 白桦脂酸体外抗肿瘤的活性和机制 |
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| 引用本文： | 王开祥,张慧,郑克岩,田家川,由宇润,孙可,费晓方. 白桦脂酸体外抗肿瘤的活性和机制[J]. 吉林大学学报(理学版), 2009, 47(3): 622-627 |
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| 作者姓名： | 王开祥 张慧 郑克岩 田家川 由宇润 孙可 费晓方 |
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| 作者单位： | 吉林大学,生命科学学院,长春130021;辽宁中医药大学,药学院,辽宁,大连,116600;吉林大学,化学学院,长春,130021;国家新药沈阳安全评价研究中心,沈阳,110021 |
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| 摘 要： | 研究白桦脂醇（Betulin）和白桦脂酸（Betulinic Acid, BA）对3种癌细胞株的增殖抑制作用以及白桦脂酸诱导MCF 7细胞毒的分子机制. 应用结晶紫染色方法对白桦脂醇和白桦脂酸对肿瘤细胞的生长抑制作用进行筛选; 应用RT-PCR技术检测MCF 7细胞p21 mRNA, p53 mRNA, Bcl 2 mRNA和Bax mRNA的表达. 结果表明, 白桦脂醇和白桦脂酸有显著抑制肿瘤细胞生长的作用, 且有浓度依赖性; 白桦脂酸能够诱导MCF 7细胞凋亡, 并诱导其p21 mRNA和p53 mRNA表达增高, 但对其Bcl 2 mRNA和Bax mRNA的表达无影响.
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| 关 键 词： | 白桦脂醇 白桦脂酸 细胞周期 凋亡 |
| 收稿时间： | 2008-10-27 |
Cytotoxicity and Molecule Mechanism of Betulinic Acid |
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| WANG Kai-xiang,ZHANG Hui,ZHENG Ke-yan,TIAN Jia-chuan,YOU Yu-run,SUN Ke,FEI Xiao-fang. Cytotoxicity and Molecule Mechanism of Betulinic Acid[J]. Journal of Jilin University: Sci Ed, 2009, 47(3): 622-627 |
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| Authors: | WANG Kai-xiang ZHANG Hui ZHENG Ke-yan TIAN Jia-chuan YOU Yu-run SUN Ke FEI Xiao-fang |
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| Affiliation: | 1. College of Life Science, Jilin University, Changchun 130021, China;2. Department of Drug, Liaoning University of Traditional Chinese Medicine, Dalian 116600, Liaoning Province, China;3. College of Chemistry, Jilin University, Changchun 130021, China;4. National New Drug Safety Estimate Center, Shenyang 110021, China |
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| Abstract: | To investigate the anti-proliferative effect of betulin and betulinic acid on 3 kinds of human cancer cells and the mechanism of betulinic acid induced cytotoxicity in MCF-7 cell line,crystal violet dyeing was used to screen the cytotoxicity effect of betulin and betulinic acid in the cancer cells.RT-PCR was operated to determine the expression of p21 mRNA,p53 mRNA,Bcl-2 mRNA and Bax mRNA.This study shows that the growth of tumor cells is significantly inhibited by betulin and betulinic acid in dose-depende... |
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| Keywords: | betulin betulinic acid cell cycle apoptosis |
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