| 拟南芥LRR-RLK亚家族基因At1 g09970突变体鉴定及表型观察 |
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| 引用本文： | 郭立琳,邓沛怡,陈奇辉,栾升,李东屏.拟南芥LRR-RLK亚家族基因At1 g09970突变体鉴定及表型观察[J].湖南师范大学自然科学学报,2006,29(2):80-83. |
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| 作者姓名： | 郭立琳 邓沛怡 陈奇辉 栾升 李东屏 |
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| 作者单位： | 湖南师范大学生命科学学院,中国,长沙,410081 |
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| 基金项目： | 国家自然科学基金资助项目(30370794)，湖南省教育厅青年基金资助项目(0413038) |
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| 摘 要： | LRR-RLK是植物类受体蛋白激酶家族(RLK)中最大的亚家族.该亚家族基因在调控植物体生长发育和抗逆性方面发挥重要作用.At1g09970是LRR-RLK亚家族成员.通过PCR和RT-PCR在DNA和RNA水平上筛选鉴定At1g09970对应的T-DNA插入失活的突变体.对萌发和萌发后阶段进行突变体的表型分析结果表明,At1g09970突变体在萌发后对NaCl敏感,暗示该基因与植物的抗盐胁迫有关.
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| 关 键 词： | At1g09970 LRR-RLK NaCl胁迫 |
| 文章编号： | 1000-2537（2006）02-0080-04 |
| 收稿时间： | 2006-01-03 |
| 修稿时间： | 2006年1月3日 |
Identification of LRR-RLK Gene At1 g09970 Homozygous Mutants and Their Phenotypic Observation in Arabidopsis |
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| GUO Li-lin,DENG Pei-yi,CHEN Qi-hui,LUAN Sheng,LI Dong-ping.Identification of LRR-RLK Gene At1 g09970 Homozygous Mutants and Their Phenotypic Observation in Arabidopsis[J].Journal of Natural Science of Hunan Normal University,2006,29(2):80-83. |
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| Authors: | GUO Li-lin DENG Pei-yi CHEN Qi-hui LUAN Sheng LI Dong-ping |
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| Abstract: | LRR-RLK(Leucin-rich-repeat receptor like kinase) is the largest subfamily in plant RLK family,and some genes of which show that they play very important roles in the regulation of plant development and stressresistance.At1g09970 is a nember of LRR-RLK genes.Three homozygous T-DNA mutants were identified at DNA and RNA levels by PCR and RT-PCR.The phenotype of the mutants which were observed at the stages of germination and postgermination showed that At1g09970 mutants were sensitive to NaCl and it suggested that this gene may participate in salt-stress resistance process in plant. |
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| Keywords: | At1g09970 LRR-RLK NaCl stress |
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