| 新型亲和活性配基及其纯化功能 |
| |
| 引用本文： | 高昆玉,杨利,富洵.新型亲和活性配基及其纯化功能[J].大连理工大学学报,1991(4). |
| |
| 作者姓名： | 高昆玉 杨利 富洵 |
| |
| 作者单位： | 大连理工大学化工学院
(高昆玉，杨利)，大连理工大学化工学院(富洵) |
| |
| 摘 要： | 以Ｄ．Ｓ．Ｄ．酸为桥基，氯三嗪为活性基，对－氨基苯甲酸、对－氨基苄基膦酸、对－氨基苯磺酸为尾基合成了三只对称型无色活性配基，其结构经波谱法及色谱法验证；比较了它们对碱性磷酸酶的纯化功能。结果表明：以苯甲酸及苄基膦酸作尾基者效果较好，回收率达８０％以上；配基浓度及缓冲液ｐＨ值对回收率影响明显。
|
| 关 键 词： | 酶 提纯 色谱法/配基 磷酸酯酶 |
Novel symmetrical colourless reactive ligands and their selective purification of alkaline phosphotase |
| |
| Gao Kunyu,Yang Li,Fu Xun.Novel symmetrical colourless reactive ligands and their selective purification of alkaline phosphotase[J].Journal of Dalian University of Technology,1991(4). |
| |
| Authors: | Gao Kunyu Yang Li Fu Xun |
| |
| Abstract: | Three symmetrica1 co1ourless reactive ligands in which the bridge part is D. S. D. acid, the reactive group is monochlorotriazine and the terminal part is aminobenzoic, aminosulphonic or aminobenzyl phosphonic acid are synthesized. Their constitution is proved by spectroscopy and chromatography. Their selective purification of a1kaline phosphotase is investigated. The best purification for alka1ine phosphotase using the ligands with benzoic acid and benzy1 phosphonic acid being the terminal part is achieved, and the recovery yie1d is about 80%.The effect of 1igand concentration and pH of equi1ibration on chromatographic behavior is obvious. |
| |
| Keywords: | enzyme purification chromatography/ligand phosphotase |
| 本文献已被 CNKI 等数据库收录！ |
