| 苏云金杆菌SD－5菌株晶体蛋白的研究 |
| |
| 引用本文： | 戴美学,严芝学,李世国,祖爱民,张红梅.苏云金杆菌SD－5菌株晶体蛋白的研究[J].山东师范大学学报(自然科学版),1995(4). |
| |
| 作者姓名： | 戴美学 严芝学 李世国 祖爱民 张红梅 |
| |
| 作者单位： | 山东师范大学生物学系，济南教育学院生物学系 |
| |
| 摘 要： | 研究了ＳＤ－５菌株伴孢晶体结构多肽组成和抗原特性，分析了氨基酸组成并进行了糖蛋白的检测．结果表明：ＳＤ－５晶体蛋白主含Ｍｗ１３５０００、Ｍｗ６５０００两条多肽，另见有－Ｍｗ２４００００的弱带，不同方法处理的样品，电泳图谱有一定差异；ＳＤ－５晶体蛋白抗血清与其晶体抗原形成两条沉淀线，而与７２１６、ＨＤ－１形成一条沉淀线；ＳＤ－５晶体蛋白由１８种氨基酸组成，不含糖蛋白成分．
|
| 关 键 词： | 苏云金杆菌 SD－5 伴孢晶体 |
STUDIES ON THE PARASPORAL CKYSTAL PROTEIN OF BACILLUS THURINGIENSIS SD-5 |
| |
| Dai Meixue,Yan Zhixue, Li Shiguo, Zu Aimin, Zhang Hongmei.STUDIES ON THE PARASPORAL CKYSTAL PROTEIN OF BACILLUS THURINGIENSIS SD-5[J].Journal of Shandong Normal University(Natural Science),1995(4). |
| |
| Authors: | Dai Meixue Yan Zhixue Li Shiguo Zu Aimin Zhang Hongmei |
| |
| Abstract: | Through studying the structural polypeptide and antigen property of parasporalcrystal protein of SD-5,analysing its amino acid component ,and examing the sugaryprotein,wegot the following resuIts :The crystal protein of SD-5 contains mainly two polypeptides of Mw135 000 and Mw65 000;There were some differences in SDS-PAGE between the samples treatedwith different methods;The antiserum against crystal protein of SD-5 formed two precipitatinglines whenit reacted with the antigen of crystal protein of SD-5 ,but only one respectively with7216 and HD-1;The crystal protein of SD-5 contains 18 species of amino acid and had no sug-aryproteIn in it. |
| |
| Keywords: | Bacillus thuringiensis SD-5 parasporal crystal |
| 本文献已被 CNKI 等数据库收录！ |
|
