Sequence analysis of the gene correlated with cytoplasmic male sterility (CMS) in rape-seed (Brassica napus) Polima and Shaan 2A |
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Authors: | WANG Yongfei Ma Sanmei WANG Ming ZHENG Xueqin GU Mao HU Shengwu |
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Institution: | WANG Yongfei, Ma Sanmei, WANG Ming, ZHENG Xueqin, GU Mao & HU Shengwu Department of Biology, Yantai Normal University, Yantai 264025, China; Northwest Science and Technology University of Agriculture and Forestry, Yangling 712100, China; National Key Biotechnology Laboratory for Tropical Crops, Haikou 571101, China; Shengzhen Vocational Technology College, Shengzhen 518005, China |
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Abstract: | orf224 is a CMS-related mitochondrial gene discovered in Polima cytoplasm. Shaan 2A CMS line is the parent of the first rapeseed hybrid cultivar Qinyou No. 2that has been grown in many regions of China. In this work,genomic DNA of Polima CMS line and Shaan 2A CMS line were used as templates, two primers of specific oligonucleoamplification fragments were cloned into pGEM-T Easy vectors and DNA sequences were determined. The CMSassociated gene, orf224-1 present in Shaan 2A CMS line, has a sequence highly homologous to the orf224 of the Polima CMS line, except for one nucleotide at position +398. There were only one base (AAC→AGC) and one amino acid (Asn →Ser) differences between the two. The homologies of the two sequences in nucleotide and. amino acid were 99.9 % and 99.6%, respectively. It is concluded that orf224 in Polima CMS line and orf224-1 of Shaan 2A CMS line are the allele at the same locus in mitochondria.`` |
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Keywords: | rapeseed (Brassica napus) Polima CMS line Shaan 2A CMS line cytoplasmic male sterility (CMS) orf224 orf224-1 |
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