可溶性人类白细胞抗原-G在重度子痫前期患者胎盘组织培养液中的表达

为了检测妊娠晚期重度子痫前期患者和正常妊娠妇女胎盘组织培养液中可溶性人类白细胞抗-G(soluble human leukocyte antigen-G,sHLA-G)的表达水平,探讨sHLA-G在子痫前期发病中的作用,选择了2008年10月至2009年4月第四军医大学唐都医院重度子痫前期患者20例(重度子痫前期组),正常足月妊娠者20例(正常足月妊娠组),用酶联免疫吸附法(ELISA)检测胎盘组织培养液中sHLA-G的表达水平.经检测重度子痫前期组胎盘组织培养液中sHLA-G的表达水平(26.78 ± 5.17 U/ml)明显低于正常妊娠组(53.64 ± 28.85U/ml)(P <0.05).实验表明,sHLA-G在重度子痫前期患者胎盘组织培养液中表达水平均明显降低,可能与子痫前期发病有关.

Expression Of Soluble Human Leukocyte Antigen-G In Placenta Culture Supernatant Of Patients With Severe Preeclampsia

Objective: we sought to evaluate the levels of sHLA-G protein in placenta culture supernatant of patients with severe preeclampsia during the third trimester to explore its significance in preeclampsia. Methods: Enzyme linked immunosorbent assay (ELISA) was used to detect the level of sHLA-G in placenta culture supernatant in severe preeclampsia group (n = 20) and in normal pregnancy group (n = 20) from October of 2008 to April 2009 in Tangdu hospital. Results: The placenta culture supernatant level of sHLA-G in severe preeclampsia group was lower than that in normal pregnancy group (P < 0.05). Conclusion: A reduced level of sHLA-G protein in placenta culture supernatant of patients with severe preeclampsia during the third trimester may associate with the pathogenesis of preeclampsia.