盐胁迫下盐藻特异表达基因片段的克隆

盐碱、干旱、极端温度等逆境条件是影响植物生长的重要因素。以抑制消减杂交及差式库的筛选对盐藻（Dunaliella salina)在高渗胁迫下特异表达基因片段进行了克隆。比较长期（1周，LS）及短期（16h,SS)NaCl胁近条件下基因表达情况，前者克隆到2个特异表达片段，后者也有2个。通过测序及Gene Bank中进行同源搜索，均未有可靠的同源性结果。可以初步认为，以上得到的4个cDNA可能是新基因或此片段不在保守区内。

Cloning of Expressing cDNA Segments in Dunaliella Salina ( Chlorophaceae ) Under Salt Stress Expression of Human RP2 Gene in Drosophila Embryo Cells in vitro

Adversity, such as salinity, aridity and extreme temperature, is an important factor to affect plant growth. In the present study, some cDNAs of the specially expressing genes of Dunaliella salina under hyperosmotic shock were cloned by suppression subtractive hybridization (SSH) and differentially screening of subtracted library. The differentially expression genes under salt stress for a longer phase (1 week, LS) were compared to those for a shorter phase( 16 hour, SS). Two cDNA clones were obtained from the former treatment and 3 clones from the latter treatment. After sequencing and homogeneity search in GeneBank, no reliable homogeneity results were found. It seems tentatively that they are probably novel genes or the fragments which are not located in the conservation domain.