| 重组酵母Saccharom yces cerevisiae蛋白激酶Sch9的分泌表达与活性鉴定 |
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| 引用本文： | 刘昕,吕逢春,杜林方,段伟,刘科.重组酵母Saccharom yces cerevisiae蛋白激酶Sch9的分泌表达与活性鉴定[J].四川大学学报(自然科学版),2011,48(5):1197-1203. |
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| 作者姓名： | 刘昕 吕逢春 杜林方 段伟 刘科 |
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| 作者单位： | 1. 四川大学生命科学学院,成都,610065
2. 澳大利亚维多利亚州,迪肯大学医学院3217 |
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| 基金项目： | 中国国家自然科学基金（30671181）； 中国国家重点技术研发项目（2006BAF07B01） |
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| 摘 要： | 为了深入研究蛋白激酶Sch9的生物化学特性,在Pichia pastoris酵母KM71H菌种中分泌表达并纯化了重组Sch9蛋白.进一步通过体外磷酸化实验分析了重组Sch9蛋白的蛋白激酶活性.通过重组蛋白质工程的手段初步研究了Sch9蛋白的生物化学和酶学特性.
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| 关 键 词： | 酵母 蛋白激酶 表达 |
Secretory expression and characterization of recombinant Saccharomyces cerevisiae protein kinase Sch9 |
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| LIU Xin,LU Feng-Chun,DU Lin-Fang,DUAN Wei,LIU Ke.Secretory expression and characterization of recombinant Saccharomyces cerevisiae protein kinase Sch9[J].Journal of Sichuan University (Natural Science Edition),2011,48(5):1197-1203. |
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| Authors: | LIU Xin LU Feng-Chun DU Lin-Fang DUAN Wei LIU Ke |
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| Institution: | College of Life Sciences, Sichuan University;College of Life Sciences, Sichuan University;College of Life Sciences, Sichuan University;School of Medicine, Deakin University, Waurn Ponds, Vic. 3217, Australia;College of Life Sciences, Sichuan University |
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| Abstract: | To facilitate the biochemical characterization of Sch9,the recombinant Sch9 protein was prepared in Pichia pastoris strain KM71H.An N-terminally polyhistidine tagged protein kinase Sch9 was produced and purified via affinity chromatography.After confirming the identity of the recombinant Sch9 protein,was its enzymatic activity further analyzed by an in vitro kinase assay.Our study provides the first insight into the biochemical properties and enzymatic activity of Sch9 via a recombinant protein engineering approach. |
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| Keywords: | Saccharomyces cerevisiae protein kinase expression |
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