ELISA方法检测恒河猴血清中抵抗素

目的用ELISA检测试剂盒,探索一种恒河猴血清抵抗素（Resistin）定量的快速检测方法,初步建立各年龄段恒河猴血清抵抗素的正常参考值。方法选择用于人抵抗素检测的ELISA定量检测试剂盒,对恒河猴血清进行抵抗素的测定;按年龄段将实验猴分组,幼年组、成年组和老年组,每组10只,制备血清,检测抵抗素,并进行统计分析,初步建立各年龄段恒河猴血清抵抗素的正常参考值。结果用ELISA定量检测试剂盒测定恒河猴血清抵抗素数值稳定,幼年组、成年组和老年组的血清抵抗素正常参考值分别为（6 510±1 730）pg/mL、（5 880±1 250）pg/mL和（1 760±920）pg/mL,有随年龄增长而逐步降低的趋势,老年组极显著低于幼年组和成年组（P〈0.01）。结论人抵抗素ELISA检测试剂盒可以作为一种对恒河猴血清抵抗素定量的临时快速的检测方法,且随着年龄的增长其血清抵抗素正常参考值有逐步降低趋势。

Determination of Serum Resistin in Rhesus Monkey by ELISA

Objective To develop the reference values for serum resistin in rhesus monkeys at various ages using ELISA,a rapid quantitative method.Methods The rhesus monkey were divided into three groups(young,adult and elder groups,10 each group).Sera were collected and resistin was determined using human-resist in ELISA quantitative detection kit.The OD450 values were analyzed by statistic methods.Results The OD450 values decreased with aging.The mean value was(6 510±1 730) pg /mL in young group,(5 880±1 025) pg /mL inadult group and(1 760±920) pg /mL in elder group.The value of elder group was significant lower than that ofthe young group(P<0.01).Conclusion The human-resistin ELISA kit is a quantitative and rapid detection toolfor resistin determination.The reference values of serum resistin in rhesus monkeys are developed,and they decreased with aging.