蛋白芯片技术联合定量检测妇科肿瘤标志物的研究

目的：研究 C12联合检测多种肿瘤标志物对妇科恶性肿瘤诊断的意义。方法选取100例妇科良性病变、73例健康女性和病理确诊的81例妇科恶性肿瘤（卵巢癌、子宫颈癌、子宫内膜癌）病例的血清标本，利用 HD-2001A蛋白芯片仪进行12种肿瘤标志（包括 CA199，NSE，CEA，CA242，CA125，CA153，AFP，Ferritin，Free-PSA，PSA， HGH and β-HCG）的联合定量检测，对比 C12芯片检测系统检测妇科恶性肿瘤与良性病变及正常对照组的差异性。结果妇科恶性肿瘤组中 CA199、CEA 水平及阳性率均高于良性病变组和正常对照组，统计学差异显著（p〈0.05）。卵巢癌中 CA199和 CA125、子宫颈癌和子宫内膜癌中 CEA 水平高于正常对照组，差异有显著性（p〈0.05）。卵巢癌中 CA199和 CA125联合检测能提高阳性率。结论利用 C12联合检测多种肿瘤标志物可以提高恶性肿瘤诊断的准确性，其中卵巢癌中 CA199和 CA125、子宫颈癌和子宫内膜癌中 CEA 肿瘤标志物蛋白芯片检测对筛查妇科肿瘤高危人群具有重要意义。

Combined detection of multiple biomarkers in gynecological tumors by protein chip technology

The present study aims to explore the significance of the combined detection of multiple serum tumor-markers using protein biochip technique in patients with gynecological tumors. The serum levels of 12 tumor markers, including CA19-9, NSE ,CEA, CA242 ,CA125, CA153, AFP, ferritin, free-PSA, PSA, HGH and β-HCG were measured using the multiple-tumor marker protein chip system （HD-2001A） in 81 cases of gynecologic tumors（including ovarian cancer, cervical cancer, endometrial cancer）, 100 cases of benign lesions and 73 cases of healthy adults. The differences among the gynecological malignancy group, benign lesions group and normal control group were analyzed. The serum levels and positive rates of CEA and CA199 in patients with gynecological malignancy were significantly higher than those in patients with benign lesions and healthy adults （p〈 0.05）. The serum levels of CA199 and CA125 in cases with ovarian cancer were significantly higher than those of the normal control group（p〈0.05）, and the serum levels of CEA in cases with uterine cervical cancer and endometrial carcinoma were also significantly higher than those of the normal control group（p〈0.05）. The combined detection of CA199 and CA125 can improve the positive rate of ovarian cancer. Combined detection of multiple serum tumor-markers using protein biochip technology can significantly increase the diagnostic sensitivity for gynecological malignancy. The combined detection of CA199 and CA125 play important roles in the positive detective rates in population with high risk of gynecological malignancy.