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6B1细胞的低频弱电磁场效应研究
引用本文:刘曼,王星,周爱华,于海鹰,庞利君,朱明尧,王磊光. 6B1细胞的低频弱电磁场效应研究[J]. 南开大学学报(自然科学版), 2000, 33(1): 110-113
作者姓名:刘曼  王星  周爱华  于海鹰  庞利君  朱明尧  王磊光
作者单位:南开大学物理科学学院,生物物理科学与技术系!天津,300071,南开大学物理科学学院,生物物理科学与技术系!天津,300071,南开大学物理科学学院,生物物理科学与技术系!天津,300071,南开大学物理科学学院,生物物理科学与技术系!天津,300071,南开大学物理科学学院,生物物理科学与技术
基金项目:国家自然科学基金!( 3950 0 0 33)资助项目
摘    要:以小鼠6B1骨髓瘤细胞为效应靶细胞,采用细胞化学(MTT),免疫酶标(ELISA)等方法,研究了低频弱电磁场对6B1细胞代谢的影响。结果表明:(1)1mV/cm的低频电场(10~100Hz)对6B1细胞的增殖无明显作用,而1mV/cm,50Hz电场对6B1细胞乙肝表面抗体的分泌有显著促进,提示在此条件下电场对6B1细胞膜的外侧存在某种特异性作用;(2)0.8T、30Hz的弱磁场对6B1细胞的增殖有

关 键 词:6B1细胞增殖 弱电磁场效应 低频 骨髓瘤
修稿时间:1999-04-19

EFFECTS OF EXTREMELY LOW FREQUENCY WEAK ELECTROMAGNETIC FIELDS ON 6B1 MYELOMA CELLS
Hermann Berg,Liu Man,Wang Xing,Zhou Aihua,Yu Haiying,Pang Lijun,Zhu Mingyao,Wang Leiguang,Hermann Berg. EFFECTS OF EXTREMELY LOW FREQUENCY WEAK ELECTROMAGNETIC FIELDS ON 6B1 MYELOMA CELLS[J]. Acta Scientiarum Naturalium University Nankaiensis, 2000, 33(1): 110-113
Authors:Hermann Berg  Liu Man  Wang Xing  Zhou Aihua  Yu Haiying  Pang Lijun  Zhu Mingyao  Wang Leiguang  Hermann Berg
Abstract:In this article, 6B1 myeloma cells of small rat were chosen as target cells, MTT colorimetry and ELISA method were used to study the effects of extremely low frequency (ELF) weak electromagnetic fields (EMFs) on the metabolism of 6B1 cells. The results show that: (1) 1mV/cm (10~100Hz) weak electric field (EF) has no significant effect on proliferation and activity of succinate dehydrogenase of 6B1 cells. However, 1mV/cm (50Hz) EF significantly promotes the HBs Ab (Hepatitis B surface antibody) secretion of 6B1 cells, pointing out that EF has some distinctive effect on the outerface of 6B1 cell membrane under this condition; (2) 0.8mT (30Hz) weak magnetic field (MF) significantly inhibits the proliferation of 6B1 cells (about 40%), but this effect is small at other frequencies.
Keywords:B1 cells  MTT  ELISA
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