豇豆SSR标记在豌豆中的可转移性与应用初探

利用豇豆SSR引物筛选在豌豆具通用性的SSR引物。13对豇豆SSR引物中检测出8对引物在豌豆中可扩增,其中5对SSR引物表现出多态性,进而分析11个豌豆品种和5个豇豆品种的遗传多样性与遗传关系。结果表明:平均等位基因数、平均有效等位基因数、平均Shannon指数,在豌豆品种群中分别为2.00、1.64、0.55,豇豆品种群中分别为2.00、1.80、0.53,上述遗传参数反映出供试豌豆品种和豇豆品种具有中等水平的遗传多样性;利用UPGMA聚类分析,能将豌豆品种群和豇豆品种群分为两类,且每个品种群内的各个体之间能进行区分,尤其是两个豌豆品种亚群的聚类结果与其地理来源基本一致。

Analysis and Application of Transferable SSR Molecular Markers from Cowpea(Vigna unguiculata)to Pea(Pisum sativum)

Aimed to screen out transferable SSR （simple sequence repeat） molecular markers from cowpea （Vigna unguiculata） to pea （Pisum sativum）. Using thirteen cowpea SSR primer pairs, eight primer pairs were successfully cross-amplified in pea. Five of the eight primer pairs exhibited polymorphism, and then to study the genetic variation of eleven pea cultivars and five cowpea cuhivars. Results showed that genetic parameters of the average number of alleles, the average effective number of alleles and the average Shannon＇ s index are 2. 00, 1.64, 0.55 in the pea cultivar group, and 2.00, 1.80, 0. 53 in the cowpea cuhivar group, respectively. These indicated that a moderate level of genetic diversity was revealed in the two cultivar groups. In addtion, UPGMA clustering showed that the pea cultivar group and the cowpea cuhivar group clustered into their own branches, respectively. Within each group, most of the samples can be distinguished from each other. Especially, within the pea cultivar group, all the samples can be divided into two subgroups, which are consistent with their geographical origin.