早期母子隔离应激对大鼠学习记忆能力的影响

目的探讨早期母子隔离应激对幼鼠学习记忆能力的影响。方法30只SD新生大鼠用于实验，实验随机分成3组，正常对照纽、母子隔离15分钟纽、母子隔离3小时纽。在生后40天，三组大鼠进行Morris水迷宫及记录海马脑片长时程增强电位（LTP）实验。结果Morris水迷宫测试，与正常组比较母子隔离3h组大鼠寻找平台需要的潜伏期延长（P〈0．05），而母子隔离15分钟组大鼠寻找平台需要的潜伏期与正常组比较则无明显变化（P〉0．05）；海马脑片长时程增强电位提示：正常对照组条件刺激（CS）前后fEPSP斜率变化率为：64．7±22．9％；母子隔离3h组为：35．3±14．2％，低于正常组（P〈0．01）；母子隔离15min组为：66．3±29．7％，与正常组比较无显著性意义（P〉0．05）。结论幼鼠生后早期过度的应激导致大鼠的学习记忆能力减弱。

Effect of Early Maternal Separation on Learning and Memory Ability of Rats

Objective To investigate the effect of early Maternal separation on learning and memory ability of rats. Methods 30 newborn SD rat pups were randomly divided into 3 groups ： normal group, isolation for 15 minutes group, isolation for 3 hours group. 40 days after birth, three groups of rats were recorded by Morris water maze and hippocampal slices of the long- term potentiation （LTP） experiment. Results Morris water maze test, compared with normal rats of maternal separation 3 h incubation period, required to extend the search platform （ p 〈 0. 05 ）, while there is no significant change between maternal isolation 15 minutes rats and normal group （ p 〉0.05 ）. The changes of bctbre and after of CS in fEPSP slope control group was 64.7 ±22.9% ; early maternal separation 3 h group was 35.3± 14.2% ,which is lower than the nor- mal group （P 〈 0. 01 ） ;early maternal separation 15 rain group ：66.3 ±29.7% compared with normal group has no significant difference （P 〉 0. 05 ）. Conclusion Early excessive stress decreases the ability of learning and memory at adult rats.