复方木香保健酒对脾虚大鼠的影响

为探究复方木香保健酒对脾虚大鼠(Rattus norvegicus)的影响，选取SPF级SD大鼠，采用皮下注射利血平结合不规则喂养构建大鼠脾虚模型，并在造模成功后设置6个处理组，每组含10只大鼠，雌雄各半，其中：正常组(N组)保持正常喂养方式，模型组(T0组)保持脾虚状态，且均不予以药物治疗；莫沙必利组(TM组)予以每1 kg体质量0.01 g的枸橼酸莫沙必利，低、中、高剂量组(T1、T2和T3组)的给药量分别为每1 kg体质量给予10.0、5.0和2.5 mL的复方木香药液(其中药物含量相当于3.00 g·mL -1的生药材)。连续给药14 d后，采集大鼠血样并进行有关血液指标的测定。结果显示：1) T0组大鼠精神萎靡、扎堆、拱背、皮毛枯槁、嗜睡等症状依然明显；T1、T2、T3和TM组大鼠的脾虚症状均有所减轻，与T0组相比体质量增长率和食物利用率有所提高。2) 经过药物治疗，脾虚大鼠血液中的血小板计数得到提升，白细胞计数和总蛋白质量浓度与正常大鼠无明显差异；但血红蛋白质量浓度与红细胞计数未恢复。3) N组与T0组相比血清D-木糖质量浓度有统计学意义上的升高(p<0.05)，T3组与N组的该指标差异无统计学意义，但与T0组的该指标差异有统计学意义(p<0.05)。N组与T0组相比血清免疫球蛋白G质量浓度有统计学意义上的升高(p<0.05)，但4个药物治疗组与N组的该指标差异无统计学意义。N、T3组与T0组相比血清胃动素均有统计学意义上的升高(p<0.01)，N、T3组雄性个体间的该指标差异无统计学意义但雌性个体间的该指标差异具有统计学意义(p<0.05)；N组与其他5组相比血清胃泌素质量浓度均有统计学意义上的升高(p<0.05)，TM、T3组与T0组相比该指标也有统计学意义上的升高(p<0.05)。N组与T0组的血清血管活性肠肽质量浓度差异具有统计学意义(p<0.01)；在雄性个体间，TM、T3组与T0组相比血清血管活性肠肽质量浓度均有统计学意义上的升高(p<0.05)，但与N组相比该指标没有统计学意义上的差异；在雌性个体间，T3组与T0、N组相比血清血管活性肠肽质量浓度分别有统计学意义上的升高 (p<0.01)和降低(p<0.05)。N、T0组雌性大鼠的淀粉酶浓度差异具有统计学意义(p<0.05)，N、T0组雄性大鼠的该指标差异无统计学意义，T2、T3和TM组与N、T0组相比该指标的差异均无统计学意义。由上述结果可知，复方木香保健酒能提升脾虚大鼠的肠胃运动能力，促进脾虚大鼠消化吸收，增强脾虚大鼠免疫力，最终促进脾虚大鼠的康复。

Effect of Compound Aucklandia lappa Health Wine on Rats with Spleen Deficiency

In order to explore the effect of compound Aucklandia lappa health wine on rats (Rattus norvegicus) with spleen deficiency, SPF SD rats were selected and the rat model of spleen deficiency was constructed by subcutaneous injection of reserpine combined with irregular feeding. SPF SD rats were selected, subcutaneously injected with reserpine combined with irregular feeding to replicate the spleen deficiency model. After successful modeling, 6 treatment groups were set up, each containing 10 rats, with half male and half female, in which: the normal group (N group) maintained the normal feeding mode, and the model group (T0 group) maintained the state of splenic deficiency, and none of them was treated with medication; the mosapride group (TM group) was administered with 0.01 g of mosapride citrate per 1 kg of body mass. The dosages of low, medium, and high dose groups (T1, T2, and T3 groups) were 10.0, 5.0, and 2.5 mL of compound A. lappa health wine per 1 kg body mass, respectively (the drug content was equivalent to the mass concentration of raw medicinal materials of 3.00 g·mL -1). After 14 days of continuous administration, blood samples were collected from rats and the relevant blood indexes were measured. The results showed that: 1) the rats in the T0 group had still obvious symptoms such as depression, clustering, arched back, withered fur and lethargy; the spleen deficiency symptoms of T1, T2, T3, and TM groups of rats were alleviated, and the growth rate of body mass and food utilization rate increased compared with those in the T0 group. 2) After drug treatment, the platelet count in the blood of splenic asthenia rats was increased, and the white blood cell count and total protein mass concentration were not significantly different from those of normal rats; however, hemoglobin mass concentration and erythrocyte count were not restored. 3) Serum D-xylose mass concentration in group N was significantly higher than that in group T0 (p<0.05), and there was no significantly different between group T3 and group N, but there was significantly different between group T3 and group T0 (p<0.05).The mass concentration of serum immunoglobulin G in group N was significantly higher than that in group T0 (p<0.05), but there was no significantly different between the 4 drug treatment groups and group N. Compared with T0 group, the serum gastrin in N group and T3 group was significantly increased (p<0.01). There was no significantly different in this index among males in N group and T3 group, but there was significantly different among females in N group and T3 group (p<0.05). Serum gastrin concentration in group N was significantly higher than that in the other 5 groups (p<0.05), and the serum gastrin concentration in groups TM and T3 was also significantly higher than that in group T0 (p<0.05). The concentration of serum vasoactive intestinal peptide was significantly different between group N and group T0 (p<0.01); among male individuals, the concentration of serum vasoactive intestinal peptide in TM and T3 groups was significantly higher than that in T0 group (p<0.05), but there was no statistically significant difference in this index compared with that in N group; among female individuals, the serum vasoactive intestinal peptide concentration in T3 group was significantly higher than that in T0 group (p<0.01), and the serum vasoactive intestinal peptide concentration in T3 group was significantly lower than that in N group (p<0.05). There was a significantly different in the concentration of amylase between the female rats of group N and T0 (p<0.05), but there was no significantly different in this index between the male rats of group N and T0, and there was no significantly different in this indicator between the T2 group, T3 group, and TM group compared to the N and T0 groups. From the above results, it can be concluded that compound A. lappa health wine can enhance the gastrointestinal motility of rats with spleen deficiency, promote digestion and absorption, enhance immunity, and ultimately promote the recovery of rats with spleen deficiency.