水蓼提取物的生物活性测定及挥发性成分的GC-MS分析

为探究水蓼(Polygonum hydropiper)活性成分的功能并拓展该物种的应用场景，以水蓼植株作为原料，分别用水提法和醇提法提取水蓼的挥发性物质，并用气相色谱-质谱联用技术对提取物进行成分鉴定，通过抑菌实验和抑制棉铃虫(Helicoverpa armigera)生长及存活实验探究水蓼提取物的生物活性。结果显示：从水蓼醇提物中分离出16个组分，其中有4种化合物得到鉴定；从水蓼水提物中分离出15个组分，其中有3种化合物得到鉴定；水蓼提取物具有一定程度的抑菌作用，对棉铃虫的生长发育也有一定抑制效果。研究结果提示：体积分数为95%的乙醇在提取水蓼挥发性物质方面的效果要优于水的效果，且水蓼挥发性物质的生物活性有待进一步验证。

Determination of Biological Activity of Polygonum hydropiper Extract and GC-MS Analysis of Its Volatile Components

To explore the function of the active components of Polygonum hydropiper and expand the application scene of P. hydropiper,the volatiles of P. hydropiper were extracted by water extraction and alcohol extraction respectively, and the components of the extracts were identified by gas chromatography-mass spectrometry (GC-MS), the biological activity of the P. hydropiper extracts was explored by bacteriostatic test and inhibition of growth and survival of Helicoverpa armigera.The results show that: the alcohol extract of P. hydropiper were identified 16 picks in the volatile oil of P. hydropiper isolated, among them, four compounds were identified,the water extract of P. hydropiper were identified 15 picks in the volatile oil of P. hydropiper isolated, among them, three compounds were identified;the extract of P. hydropiper had certain bacteriostatic effect and had certain restraining influence on the biological activity of H. armigera.The results suggest that: the effect of 95% ethanol in extracting the volatile compounds of P. hydropiper is better than that of water, and the biological activity of the volatile substances of P. hydropiper needs to be further verified.