非根际土壤中丛枝菌根网和碳酸钙互作对香樟幼苗氮磷养分的影响

为考察非根际土壤中丛枝菌根网(后简称菌根网)和碳酸钙互作对香樟(Cinnamomum camphora)幼苗氮磷养分的影响，模拟构建了植物根际和非根际隔室装置，采用尼龙网隔离实现根际与非根际隔室菌根网互联，在根际隔室中种植香樟幼苗并接种丛枝菌根真菌，在非根际隔室施加或未施加外源碳酸钙，培养期结束后测定植物生物量和氮、磷含量。结果显示：菌根网对香樟幼苗植株生物量，氮、磷摄取量及氮磷比有明显影响；施加碳酸钙明对幼苗植株氮含量、氮摄取量及氮磷比有明显的影响；在施加碳酸钙后菌根网明显提升了幼苗植株生物量，氮含量及氮、磷摄取量，叶片氮、磷摄取量和根的氮磷比；在菌根网存在时施加碳酸钙可明显提高植株氮含量、根和叶的氮摄取量以及根的氮磷比；菌根网与碳酸钙的交互作用明显影响了幼苗植株氮含量，氮、磷摄取量和氮磷比，但对幼苗植株生物量无明显影响。研究结果提示非根际喀斯特土壤中互联菌根网和碳酸钙相互作用能够促进植物对氮磷养分的吸收利用。

Effects of Cirtnamomum camphora Seedlings on Nutrients of Nitrogen and Phosphorus via Arbuscular Mycorrhizal Networks Interacting Calcium Carbonate in Non-Rhizosphere Soil

To investigate the effects of the interaction of arbuscular mycorrhizal networks and calcium carbonate on the nitrogen and phosphorus nutrients of Cirtnamomum camphora seedlings in non-rhizosphere soil. The plant rhizosphere and non-rhizosphere compartment devices were simulated and constructed, the nylon meshes isolation was used to realize the interconnection of mycorrhizal networks between rhizosphere and non-rhizosphere compartments, the rhizosphere compartment was planted with Cirtnamomum camphora seedlings with arbuscular mycorrhizal fungi, the non-rhizosphere compartment was applied to add or not add calcium carbonate. After the cultivation period, the biomass, the nutrient content of nitrogen and phosphorus of the plant were determined. The results showed that mycorrhizal networks had a significant impact on the biomass, the nitrogen and phosphorus acquisition, and the nitrogen/phosphorus ratio of camphor tree seedlings; the application of calcium carbonate had a significant impact on the nitrogen content, nitrogen acquisition, and nitrogen/phosphorus ratio of seedling plants; after adding calcium carbonate, the mycorrhizal networks significantly increased the biomass, nitrogen content, nitrogen and phosphorus acquisition of seedling plants, the nitrogen and phosphorus acquisition of leaves, and the nitrogen/phosphorus ratio of roots; applying calcium carbonate in the presence of mycorrhizal networks can significantly increase the nitrogen content of seedling plants, the nitrogen acquisition of roots and leaves, and nitrogen/phosphorus ratio of roots. The interaction of mycorrhizal networks and calcium carbonate significantly affected the nitrogen content, the nitrogen and phosphorus acquisition, the nitrogen/phosphorus ratio of seedlings but did not for the biomass of seedling plants. The results suggest that the interaction of arbuscular mycorrhizal networks and calcium carbonate can promote the absorption and utilization of nitrogen and phosphorus by plants in karst non-rhizosphere soil.