Comparison of SXT and R391, two conjugative integrating elements: definition of a genetic backbone for the mobilization of resistance determinants |
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Authors: | J W Beaber V Burrus B Hochhut M K Waldor |
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Institution: | (1) Division of Geographic Medicine/Infectious Diseases, New England Medical Center and Tufts University School of Medicine and Howard Hughes Medical Institute, NEMC 041, 750 Washington St., Boston, Massachusetts 02111 (USA), Fax: +16176365292, e-mail: mwaldor@lifespan.org, US |
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Abstract: | The SXT element (SXT) is becoming an increasingly prevalent vector for the dissemination of antibiotic resistances in Vibrio cholerae. SXT is a member of a larger family of elements, formerly defined as IncJ plasmids, that are self-transmissible by conjugation
and integrate site-specifically into the host chromosome. Comparison of the DNA sequences of SXT and R391, an IncJ element
from Providencia rettgeri, indicate that these elements consist of a conserved backbone that mediates the regulation, excision/integration and conjugative
transfer of the elements. Both elements have insertions into this backbone that either confer the element-specific properties
or are of unknown function. Interestingly, the conserved SXT and R391 backbone apparently contains hotspots for insertion
of additional DNA sequences. This backbone represents a scaffold for the mobilization of genetic material between a wide range
of Gram-negative bacteria, allowing for rapid adaptation to changing envi
ronments.
RID="*"
ID="*"Corresponding author. |
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Keywords: | , SXT, conjugative transposon, R391, gene transfer, Vibrio cholerae, comparative genomics, |
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