| PNA-磁性纳米人工切断试剂定位水解切断DNA |
| |
| 引用本文： | 杨旭石,王锋,沈鹤柏,朱龙章.PNA-磁性纳米人工切断试剂定位水解切断DNA[J].上海师范大学学报(自然科学版),2007,36(2):57-60. |
| |
| 作者姓名： | 杨旭石 王锋 沈鹤柏 朱龙章 |
| |
| 作者单位： | 上海师范大学生命与环境科学学院,上海,200234 |
| |
| 摘 要： | 制备了以二氧化硅包裹的磁性纳米粒子（MNPs）为载体，PNA（肽核酸）为识别系统，Ce（Ⅳ）／EDTA配合物为切割系统的PNA-磁性纳米切割试剂．采用TEM对制备的MNPs进行了表征，通过IP—RP—HPLC对切断产物进行了分析．结果表明，该PNA-磁性纳米人工切断试剂可以成功地结合目标DNA，并对其进行定位切断．此外，磁性纳米粒子的引入，使得切断产物的分析更加简单、快捷．
|
| 关 键 词： | 磁性纳米粒子 DNA切断 |
| 文章编号： | 24366160 |
| 修稿时间： | 12 12 2006 12:00AM |
Synthesis of magnetic nanoparticle-based artificial cleavage reagent for sequence-specific hydrolysis of single stranded DNA |
| |
| YANG Xu-shi,WANG Feng,SHEN He-bo,ZHU Long-zhang.Synthesis of magnetic nanoparticle-based artificial cleavage reagent for sequence-specific hydrolysis of single stranded DNA[J].Journal of Shanghai Normal University(Natural Sciences),2007,36(2):57-60. |
| |
| Authors: | YANG Xu-shi WANG Feng SHEN He-bo ZHU Long-zhang |
| |
| Institution: | College of Life and Environment Sciences ,Shanghai Normal University, Shanghai 200234, China |
| |
| Abstract: | A novel magnetic nanoparticles(MNPs)-based artificial cleavage reagent for single-stranded DNA(ssDNA) was synthesized by binding molecular scissor of Ce(IV)/EDTA complex on the surface of MNPs via sequence-recognizing moiety(PNA).The average size of the MNPs was determined around 100 nm by TEM.Its cleavage ability for ssDNA was investigated by HPLC,which revealed that the target ssDNA could be sequence-specifically hydrolyzed.Furthemore,MNPs greatly facilitate the isolation of the cleaved product,showing good potential in gene studies. |
| |
| Keywords: | PNA Ce |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《上海师范大学学报(自然科学版)》浏览原始摘要信息 |
| 点击此处可从《上海师范大学学报(自然科学版)》下载免费的PDF全文 |
