| Ca~(2 )激活猪凝血酶原作用的初步研究 |
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| 作者单位： | 河北省科学院生物研究所 石家庄050081
(辛春艳，陈英珠，吴萌，籍宝霞，刘玉翠)，河北省科学院生物研究所 石家庄050081(邹岳奇) |
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| 摘 要： | 为探讨Ca2 激活猪凝血酶原的最适工作浓度和激活时间 ,以新鲜猪血为材料 ,采用HAc等电点沉淀法获取凝血酶原后 ,添加 0 .2 5mol/LCaCl2 作为凝血酶原激活剂 ,使Ca2 达到不同终浓度 ,并经过不同激活时间进行对比试验。结果显示以Ca2 单一因子激活猪凝血酶原的最适CaCl2终浓度为 0 .0 5mol/L ;最适激活时间为 1.5h。使Ca2 激活猪凝血酶原的实验条件得以确立。
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| 关 键 词： | 凝血酶 凝血酶原 激活 盐析 |
Preliminary study on the effect of Ca~(2 ) activating porcine prothrombin |
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| Authors: | XIN Chun-yan CHEN Ying-zhu WU Meng JI Bao-xia LIU Yu-cui ZOU Yue-qi |
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| Abstract: | In order to approach the optimal working concentration and activating time for porcine prothrombin activation with Ca 2 , firstly porcin prothrombin was isolated using the method of HAc isoelectric fractionation from fresh porcine blood, then 0.25mol/LCaCl 2 as activator was added in the prothrombin solution. Various final Ca 2 concentrations and different activating time were tested, and the results showed that with Ca 2 as single factor to activate porcine prothrombin, the optimal final concentration of CaCl 2 was 0.05mol/L;the optimum activating time was 1.5 hours.The experimental condition for Ca 2 activating porcin prothrombin was established. |
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| Keywords: | Thrombin Prothrombin activation Salting out |
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