培养原代小鼠组织细胞检验细胞实验室技术操作规程

目的通过培养小鼠原代组织细胞,检验本科室己建立的一套细胞实验室技术操作规程能否满足细胞培养要求。方法培养小鼠原代成骨细胞、软骨细胞、心肌细胞、肝细胞、肺部细胞、脑部细胞、骨髓间充质细胞和胚胎成纤维细胞。计算原代培养成活率、传代成活率、冷冻及复苏成活率。结果成骨细胞、软骨细胞、心肌细胞、肝细胞、肺部细胞、脑部细胞、骨髓间充质细胞、胚胎成纤维细胞原代培养成活率分别为89%、96%、100%、100%、100%、80%、100%和90%;传代成活率分别为76%、93%、100%、92%、100%、66%、80%和92%;冷冻及复苏成活率分别为100%、100%、100%、100%、100%、100%、100%和100%。结论通过计算KM小鼠8种组织细胞原代培养、传代、冷冻与复苏的成活率,证明现有的细胞室技术操作规程基本上能够保证成功培养出常规原代细胞,并能传代、冷冻和复苏。

To Test Cell Laboratory Operating Instruction by Culturing Mice Primary Histiocytes

ObjectiveTo test whether that we have builded cell laboratory operating instruction satisfy the requirement of cell culture by culturing mice primary histiocytes.Method Culture mice cells: osteoblast,cartilage cell,cardiac muscle cell,hepatic cell,lung cell,brain cell,marrow MES cell,embryo fibroblast and calculate primary culture survival rate,passage survival rate,freezing-revival survival rate.Results The primary culture survival rate of osteoblast,cartilage cell,cardiac muscle cell,hepatic cell,lung cell,brain cell,marrow MES cell,embryo fibroblast respectively: 89%,96%,100%,100%,100%,80%,100% and 90%;passage survival rate: 76%,93%,100%,92%,100%,66%,80%,92%;freezing-revival survival rate: 100%,100%,100%,100%,100%,100%,100% and 100%.Conclusion Through calculating primary culture survival rate,passage survival rate,freezing-revival survival rate of 8 kinds of mice histiocytes,it is prove that we can succeedly culture revival common primary cell by using cell laboratory operating instruction at the present stage.