Effects of nitrogen ion implantation on Ca2+ concentration and membrane potential of pollen cell |
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Authors: | Huang Zhongliansu Jing Yanping Zhu Guoli Lu Ting Zhou Hongyu Ren Haiyun |
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Institution: | (1) Key Laboratory of Cell Proliferation and Regulation Biology of the Ministry of Education, Beijing Normal University, 100875 Beijing, China;(2) Key Laboratory of Plant Physiology and Biochemistry of the Ministry of Agriculture, China Agriculture University, 100094 Beijing, China;(3) Institution of Low Energy Nuclear Physics, Beijing Normal University, 100875 Beijing, China |
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Abstract: | The effects of low energy nitrogen ion implantation on Ca2+ concentration and membrane potential of lily (lilium davidii Duch) pollen cell have been studied. The results showed that the Ca2+ concentration was increased when pollen grain was implanted by nitrogen ion with energy 100 keV and dose 1013 ions/cm2. However, the increase of Ca2+ concentration was partly inhibited by the addition of Ca2+ channel inhibitor depending on dose. And nitrogen ion implantation caused depolarization of pollen cell membrane potential.
In other words, membrane potential was increased, but the effect decreased by adding Ca2+ channel inhibitor. However, it was still significantly higher than the membrane potential of control cells. It was indicated
that the depolarization of cell membrane potential opened the calcium channel on the membrane that caused the increasing of
intracellular calcium concentration. This might be an earlier step of the effect of low energy nitrogen ion implantation on
pollen germination. |
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Keywords: | nitrogen ion implantation lily pollen calcium concentration membrane potential |
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