β2-Adrenoceptor affinity chromatography and its application in the screening of the active compounds from Semen Armeniacae Amarum

β2-Adrenoceptor （β2-AR） was purified from the rabbit lung tissue by sepherose-salbutamol affinity chromatographic column. To prepare the β2-AR stationery phase, β2-AR was evenly immobilized on the surface of macro-pore silica with e mild chemical coupling method through covalent bond. The retention properties of β2-AR stationery phase were characterized by four ligends, selbutamol sulfate, noredreneline bitartrete, adrenaline hydrochloride end proprenolol hydrochloride, to establish the β2-AR affinity chromatography. Then, the method was used to screen the active compounds from the total extracts of Semen Armeniacae Amarum. The results showed that β2-AR on the surface of the stationary phase could keep its original bioectivity end selectivity. Amygdalin retained in the chromatographic column was proved to be the active compound of the total extracts of Semen Armeniacae Amarum. Compared with the existing chromatographic screening approaches, this method showed e good stability end high selectivity. The active compounds which could interact with β2-AR in traditional Chinese medicine （TCM） could be screened efficiently by this method, providing e new way to screen the active compounds in complicated samples such as TCM.