药对川芎-羌活挥发油的气-质联用分析与化学计量学解析

采用气相色谱-质谱（GC/MS）法分离测定药对川芎-羌活、单味药川芎和羌活的挥发油成分,利用化学计量学解析法（CRM）对重叠的色谱峰进行解析,得到药对和各单味药的纯色谱曲线和质谱,通过质谱库对解析的纯组分进行定性,药对川芎-羌活、单味药川芎和羌活的定性分析结果分别为50,38和43个,占总含量的94.32%,83.19%和96.04%。实验结果表明,药对挥发油组分主要来自于单味药羌活,而化学组分种类基本上为2个单味药的加和,单味药挥发油组分的含量在药对中发生了变化。

Analysis of volatile oil in herbal pair Chuanxiong Rhizome-Notopterygium Root by gas chromatography-mass spectrometry and chemometric resolution method

Analysis of volatile components in herbal pair(HP) Chuanxiong Rhizome-Notopterygium Root(CXR-NR),single herb CXR and NR was performed with two-dimensional gas chromatography-mass spectrometry(GC/MS) data coupled with chemometric resolution method(CRM).By means of these methods two-dimensional data,38,43 and 50 volatile chemical components in essential oil of CXR,NR,and HP CXR-NR were respectively determined qualitatively and quantitatively,accounting for 83.19%,96.04%,and 94.32% total contents of essential oil of CXR,NR,and HP CXR-NR,respectively.The experimental results show that the number of volatile chemical components in CXR-NR,mainly from that of single herb NR,is almost equal to the sum of the number in the two single herbs,but the relative amounts,especially the ligustilide in CXR change.