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| 不同品种猪生长激素基因的PCR-RFLP分析 | |
| 引用本文： | 王荣梅,苏荣胜,张细权.不同品种猪生长激素基因的PCR-RFLP分析[J].韶关学院学报,2009,30(9):85-88. |
| 作者姓名： | 王荣梅 苏荣胜 张细权 |
| 作者单位： | 1. 韶关学院,农业科学系,广东,韶关,512005 2. 华南农业大学,兽医学院,广东,广州,510642 3. 华南农业大学,动物科学学院,广东,广州,510642 |
| 基金项目： | 国家重大基础研究规划项目 |
| 摘 要： | 采用PCR-RFLPs技术,对临高猪、玉山黑猪、蓝塘猪及国外猪种的生长激素基因-119～＋486区域共605 bp片段的扩增产物用Apa I限制性内切酶检测酶切位点的多态性.结果显示：Apa I酶切时,四个品种的猪都出现了3种基因型（AA、BB和AB）,等位基因B的频率以蓝塘猪最高,等位基因A的频率以玉山黑猪最高,四个猪种间基因型频率、等位基因频率的差异不显著（P＆gt;0.05）. |
| 关 键 词： | 猪生长激素 基因 ApaI PCR-RFLP |
PCR-RFLP analysis of porcine growth hormone gene in different porcine breed |
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| WANG Rong-mei,SU Rong-sheng,ZHANG Xi-quan.PCR-RFLP analysis of porcine growth hormone gene in different porcine breed[J].Journal of Shaoguan University(Social Science Edition),2009,30(9):85-88. | |
| Authors: | WANG Rong-mei SU Rong-sheng ZHANG Xi-quan |
| Institution: | 1. Department of Agriculture Science, Shaoguan University, Shaoguan 512005, Guangdong, China; 2. College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,Guangdong, China; 3. College of Animal Seienee,South China Agricultural University,Guangzhou 510642,Guangdong, China) |
| Abstract: | Apa I polymorphisms in porcine growth hormone （pGH） gene＇s a 605bp segment encompassing base - 119 through base ＋ 486 were analyzed by PCR - RFLP in Lin-gao Pig ,Yushan Black Pig,Lan-tang Pig and Foreign Pigs.The results showed that there were three genetypes（AA , BB and AB） in every porcine breed when the PCR products were cut by Apa I, BB genotype frequency of Lan-tang Pig was highest in four breeds and AA genotype frequency of Yu-shan Pig was highest in four breeds , and the difference of genotype frequencies and allele frequencies in four breeds was not significant （P〉0.05）. |
| Keywords: | PCR-RFLP |
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