化学发光法检测不同干燥方法对金银花抗氧化活性和化学成分的影响

研究不同干燥方法(微波、鼓风干燥、阴干) 对金银花的抗氧化活性和总酚、总黄酮质量分数的影响。利用鲁米诺化学发光体系，以抗坏血酸为阳性对照，以超氧阴离子(O^-2)、双氧水(H₂O₂)和羟基自由基(HO·)清除为指标，评价3种不同干燥方法对金银花的抗氧化活性的影响，并对总酚和总黄酮的质量分数进行了测定。实验结果表明：金银花具有较强的抗氧化活性；不同的干燥方法对其活性物质的质量分数以及抗氧化活性的强度均有较强的影响，其中70 ℃微波干燥10 min条件下，金银花总酚和总黄酮的质量分数最高，分别为30.40±2.75 mg/g、61.24±7.62 mg/g，并且其抗氧化活性最强，3种指标的IC-50分别为金银花原提取液浓度的9.44%、0.029%、0.12%。而自然阴干4 d的条件下，金银花的总酚和总黄酮的质量分数最低，分别为10.22±0.81 mg/g、17.50±2.28 mg/g，并且抗氧化活性最弱，3种指标的IC-50分别为金银花原提取液浓度的11.97%、0.53%、0.37%。

Impacts of different drying methods on antioxidant activity and chemical ingredients of honeysuckle by chemiluminescence detection

We addressed the impacts of different drying methods (microwave drying, hot air drying and shade drying) on the antioxidant activity and chemical ingredient of honeysuckle. We also assessed the impacts of the three drying methods on the antioxidant activity of honeysuckle and determined the content of total phenolic and total flavonoid with Luminol chemiluminescence, and superoxide anion, hydrogen peroxide and hydroxyl radical scavenging as indices. Experimental results show that honeysuckle has remarkable antioxidant activity. The three different drying methods all exhibit significant impacts on the antioxidant activity and the content of active components. Antioxidant activity of total phenolic and total flavonoid is the strongest, and their content is the highest, 30.40±2.75 mg/g and 61.24±7.62 mg/g under the conditions of microwave drying at 70 ℃ for 10 min. IC-50 of the three indices is 9.44%, 0.029% and 0.12%. Antioxidant activity of total phenolic and total flavonoid is the weakest and their contents are the lowest, 10.22±0.81mg/g and 17.50±2.28 mg/g under the condition of shade drying for 4 days. IC-50 of the three indices is 11.97%, 0.53% and 0.37% of the original extract concentration.