Spectrofluorimetric determination of tetracycline using Eu3+ chelation and cetyltrimethylammonium bromide |
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| Authors: | Huang Zu-yun Chen Zhen-hua Yin Qu-an Shen Rong-sen |
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| Institution: | (1) College of Chemistry, Wuhan University, 430072 Wuhan, China;(2) Department of Human Biological Chemistry and Genetics, The University of Texas Medical Branch, 77556-0652 Galveston, Texas, USA |
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| Abstract: | The fluorescence of tetracycline was greatly enhanced by chelation with Eu3+ in Tris-HCl buffer (pH8.5). The addition of cetyltrimethylammonium bromide, a cationic surfactant, further enhanced the fluorescence
of tetracycline-Eu3+ chelates. This spectrofluorimetric method for the determination of tetracycline yielded a linear responses in the range of
10 nmol/L–10 μmol/L. The detection limit was 5 nmol/L (or 2.22 ng/mL). The recovery of tetracycline spiked in serum at levels
of 1 and 0.09 μg/mL was 97.9% and 97.8%, respectively. This method is fast, sensitive and suitable for the determination of
tetracycline in biological specimens.
Foundation item: Supported by the National Natural Science Foundation of China
Biography: Huang Zu-yun (1963-), male, Ph D. |
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| Keywords: | tetracycline (TC) spectrofluorimetry cetyltrimethylammonium bromide (CTAB) |
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