| 烟草和MPTA对三孢布拉氏霉合成番茄红素的影响 |
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| 引用本文： | 修宇,袁其朋. 烟草和MPTA对三孢布拉氏霉合成番茄红素的影响[J]. 北京化工大学学报(自然科学版), 2009, 36(1): 81-84 |
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| 作者姓名： | 修宇 袁其朋 |
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| 作者单位： | 北京化工大学生命科学与技术学院,北京,100029;北京化工大学生命科学与技术学院,北京,100029 |
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| 摘 要： | 在三孢布拉氏霉发酵48 h添加3.6 mmol/L 2-(4-甲基苯氧)-三乙基胺(MPTA)可有效抑制96% β-胡萝卜素的合成；添加15g/L烟草可促进总类胡萝卜素的产量(170mg/L)，但其对β-胡萝卜素的合成抑制作用低于MPTA。L27（3 13）正交实验从抑制剂添加时间、添加量以及两种抑制剂的交互作用等方面考察了同时添加MPTA和烟草对番茄红素产量的影响。发酵48?h同时添加烟草10g/L与MPTA 2.2mmol/L，番茄红素的产量可达205mg/L（占总类胡萝卜素产量的94%）。
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| 关 键 词： | 番茄红素 生物合成 环化酶 抑制剂 |
| 收稿时间： | 2008-05-20 |
The application of tobacco and MPTA during lycopene production by Blakeslea trispora |
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| XIU Yu,YUAN QiPeng. The application of tobacco and MPTA during lycopene production by Blakeslea trispora[J]. Journal of Beijing University of Chemical Technology, 2009, 36(1): 81-84 |
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| Authors: | XIU Yu YUAN QiPeng |
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| Affiliation: | College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China |
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| Abstract: | The efficacy and optimization of the application of two inhibitors,tobacco and bleaching herbicide 2-(4-methylphenoxy)-triethylamine(MPTA),on lycopene productivity by Blakeslea trispora were investigated.The greatest yield of lycopene(170 mg/L)was observed when nicotine in the form of tobacco was added at 15 g/L at 48 hours old mated cultures,however,this addition did not inhibit(-carotene synthesis to the same extent as MPTA's,but resulted in 53% increase in overall carotenoid synthesis.Lycopene biosynthes... |
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| Keywords: | lycopene biosynthesis lycopene cyclase inhibitor |
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