| Mouse Karyotype Obtained by Combining DAPI Staining with Image Analysis |
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| 作者姓名： | DAI Xiaohua YANG Guangxu LIU Jingyu SONG Yunchun |
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| 作者单位： | [1]College of Life Science, Henan University, Kaifeng 475001, Henan, China [2]Department of Life Science, Western Chongqing University, Chongqing 402168, Siehuan, China [3]The Key l.aboratory of the Ministry of Education of Plant Developmental Biology, Wuhan University, Wuhan 430072, Hubei, China |
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| 基金项目： | 中国博士后科学基金;河南大学校科研和教改项目 |
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| 摘 要： | In this study, mitotic metaphase chromosomes in mouse were identified by a new chromosome fluorescence banding technique combining DAPI staining with image analysis. Clear 4＇, 6-diamidino-2-phenylindole （DAPI） multiple bands like （J-hands could be produced in mouse. The Meta- Morph software was then used to generate linescans of pixel intensity for the banded chromosomes from short arm to long arm. These linescans were sufficient not only to identify each individual chromosome but also analyze the physical sites of bands in chromosome. Based on the results, the clear and accurate karyotype of mouse metaphase chromosomes was established. The technique is therefore considered to he a new method for cytological studies of mouse.
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| 关 键 词： | 小鼠 染色体组型 DAPI染色法 图象分析 |
| 文章编号： | 1007-1202（2006）02-0441-06 |
| 收稿时间： | 2005-03-05 |
Mouse karyotype obtained by combining DAPI staining with image analysis |
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| DAI Xiaohua YANG Guangxu LIU Jingyu SONG Yunchun.Mouse Karyotype Obtained by Combining DAPI Staining with Image Analysis[J].Wuhan University Journal of Natural Sciences,2006,11(2):441-446. |
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| Authors: | Dai Xiaohua Yang Guangxu Liu Jingyu Song Yunchun |
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| Institution: | (1) College of Life Science, Henan University, 475001 Kaifeng, Henan, China;(2) Department of Life Science, Western Chongqing University, 402168 Chongqing, Sichuan, China;(3) The Key Laboratory of the Ministry of Education of Plant Developmental Biology, Wuhan University, 430072 Wuhan, Hubei, China |
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| Abstract: | In this study, mitotic metaphase chromosomes in mouse were identified by a new chromosome fluorescence banding technique combining
DAPI staining with image analysis. Clear 4′, 6-diamidino-2-phenylindole (DAPI) multiple bands like G-bands could be produced
in mouse. The Meta-Morph software was then used to generate linescans of pixel intensity for the banded chromosomes from short
arm to long arm. These linescans were sufficient not only to identify each individual chromosome but also analyze the physical
sites of bands in chromosome. Based on the results, the clear and accurate karyotype of mouse metaphase chromosomes was established.
The technique is therefore considered to be a new method for cytological studies of mouse.
Foundation item: Supported by China Postdoctoral Science Foundation (2003034496) and the Key Natural Science Foundation of Henan University
(XK03ZDSW158)
Biography: DAI Xiaohua (1978-), female, Master candidate, research direction: animal genetics research |
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| Keywords: | mouse 4' 6-diamidino-2-phenylindole (DAPI) fluorescence staining image analysis karyotype |
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