首页 | 本学科首页   官方微博 | 高级检索  
     检索      

青岛文昌鱼碱性肌球蛋白轻链MLC-alk基因片段的克隆
引用本文:张燕君,梁恺龙,黄向炜,林浴霜,张红卫.青岛文昌鱼碱性肌球蛋白轻链MLC-alk基因片段的克隆[J].山东大学学报(理学版),2002,37(2):176-179.
作者姓名:张燕君  梁恺龙  黄向炜  林浴霜  张红卫
作者单位:山东大学生命科学学院,山东,济南,250100
基金项目:国家自然科学基金 (3 9670 3 5 7,3 0 0 70 0 94),高等学校博士学科点专项科研基金
摘    要:碱性肌球蛋白轻链是构成球蛋白头部的必需分子,采用简并引物PCR方法获得青岛文昌鱼碱性肌球轻链基因片段,并对其氨基酸序列与其他生物如鸡和人的胚胎裂、骨骼肌型、平滑肌型、心肌型、非肌型等多种碱性肌球蛋白轻链基因家庭成员相应片段进行了同源性分析,均显示较高的同源性,研究结果支持青岛文昌鱼具有1个,可能也仅有1个碱性肌球蛋白轻链基因,碱性肌球蛋白轻链基因原倍增可能发生在脊椎动物与文昌鱼在进化中分支之后。

关 键 词:青岛市  MLC-alk  文昌鱼  碱性肌球蛋白轻链基因  基因克隆  同源性  PCR方法
文章编号:0559-7234(2002)02-0176-04
修稿时间:2001年3月30日

CLONING OF QINGDAO AMPHIOXUS ALKALI MYOSIN LIGHT CHAIN GENE FRAGMENT,A MOLECULE INVOLVED IN MUSCULATURE DEVELOPMENT
ZHANG Yan jun,LIANG Kai long,HUANG Xiang wei,LIN Yu shuang,ZHANG Hong wei.CLONING OF QINGDAO AMPHIOXUS ALKALI MYOSIN LIGHT CHAIN GENE FRAGMENT,A MOLECULE INVOLVED IN MUSCULATURE DEVELOPMENT[J].Journal of Shandong University,2002,37(2):176-179.
Authors:ZHANG Yan jun  LIANG Kai long  HUANG Xiang wei  LIN Yu shuang  ZHANG Hong wei
Abstract:The alkali myosin light chain genes(MLC alk)code for proteins associated with the globular head of the myosin heavy chain.Degenerate primers were designed according to the conserved sequences of MLC alk gene family and a single MLC alk gene fragment was cloned by PCR from Qindao amphioxus neurula cDNA library.The homology analysis of deduced amino acid sequence showed that Qingdao amphioxus MLC alk had high level of similarities with vertebrate MLC alk genes of embryonic,skeletal muscle,smooth muscle,cardiac muscle and non muscle types.The results show that Qingdao amphioxus contains one and probably only one MLC alk gene.Thus,duplication of an ancestral vertebrate MLC alk gene probably occurred after the vertebrate and amphioxus lineages split.
Keywords:amphioxus  alkali myosin light chain gene  cloning
本文献已被 CNKI 维普 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号