| 降胆固醇干酪乳杆菌HC12的培养条件优化 |
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| 引用本文： | 刘长建,姜波,蒋本国,闫建芳,刘秋.降胆固醇干酪乳杆菌HC12的培养条件优化[J].中央民族大学学报(自然科学版),2012,21(1):18-21,27. |
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| 作者姓名： | 刘长建 姜波 蒋本国 闫建芳 刘秋 |
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| 作者单位： | 大连民族学院生命科学学院,辽宁大连,116600 |
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| 基金项目： | 国家自然科学基金(No.30671398);NSFC-KOSEF国际合作项目(No.30711140389) |
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| 摘 要： | 干酪乳杆菌HC12具有降低培养基中胆固醇的能力,通过单因子、正交试验对其培养条件和培养基进行了优化.在MRS培养基的基础上,培养基最佳配比为酵母粉2.0%,葡萄糖2.5%,胰蛋白胨1.5%,牛肉膏1.0%;最适的起始pH值为6.0,最适温度为28℃.优化后的培养基、培养条件使干酪乳杆菌HC12的生长量(OD值)达到了1.502,去除胆固醇的量达到54.3 ug/mL.
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| 关 键 词： | 干酪乳杆菌 培养条件 OD值 胆固醇去除能力 |
Optimization of Culture Conditions of Cholesterol-lowering Lactobacillus casei HC12 |
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| LIU Chang-jian,JIANG Bo,JIANG Ben-guo,YAN Jian-fang,LIU Qiu.Optimization of Culture Conditions of Cholesterol-lowering Lactobacillus casei HC12[J].Journal of The Central University for Nationalities(Natural Sciences Edition),2012,21(1):18-21,27. |
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| Authors: | LIU Chang-jian JIANG Bo JIANG Ben-guo YAN Jian-fang LIU Qiu |
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| Institution: | (College of Life Science,Dalian Nationalities University,Liaoning Dalian 116600,China) |
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| Abstract: | Culture medium and conditions of Lactobacillus casei HC12 were optimized by single-factor and orthogonal experiments.Baced on MRS medium,several compoments of MRS medium were optimized as follows: yeast extract powder 2%,glucose 2.5%,tryptone 1.5%,beef extract 1.0%.Its optimal culture conditions were as follows : initial pH 6.0;temperature of fermentation 28 ℃.The growth(OD value) of strain HC12 was up to 1.502,and the ability of cholesterol removal reached 54.3 ug/mL in the optimum culture medium and conditions. |
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| Keywords: | Lparaplantium casei culture conditions OD value cholesterol removal ability |
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