苯妥英钠对鼠胚MDA、SOD、GSH－Px的影响

给ＮＩＨ孕鼠腹腔注射苯妥英钠（ＰＨＴ）后观察腭裂发生率为２２．４％，显示ＰＨＴ对ＮＩＨ小鼠只有致畸性，并测得胚胎内丙二醛（ＭＤＡ）值显著增高（Ｐ＜０．０５），支持ＰＨＴ致畸与自由基有关，揭示ＰＨＴ可能通过增强前列腺素合成酶活性而导致自由基生成增多，但未见ＰＨＴ对超氧化物歧化酶（ＳＯＤ）有影响．维生素Ｅ（ＶｉｔＥ）作为自由基清除剂能逆转ＰＨＴ所致ＭＤＡ值增高（Ｐ＜０．０５），而对ＳＯＤ活性无明显影响．但过量的ＶｉｔＥ与ＰＨＴ合用反而使腭裂发生率增高。

The Effect of Phenytoin on Intraembryonie MAN,SOD and GSH-Px Level of Mice

Phenytoin (PHT) was given intraperitoneally to the pregnant NIH mice, The incidence of cleft palate deformity was 22.4%, which revealed that PHT possesses teratogenicity in NIH mice. It increased the intraembryonic malondialdehyde (MDA) value significantly which supported the close relation between the PHT induced teratogenesis and free radicals (P<0.05). This also suggested that it is possible the activity of prostaglandin biosynthesis was enhanced thus increased the synthesis of free radicals. The result also showed that superoxide dismutase (SOD)activety was not much changed by PHT. As a free radical scavenger, Vitamin E (VitE) could reverse PHT-induced MDA increaseing (P<0.05), but not altered the SOD activity. On the contrary, excessive VitE with PHT together may increase the incidence of cleft palate (P<0.05),significantly.