小冠花_Coronillavaria_根瘤菌生理生化特性和共生效应的研究

小冠花根瘤菌8406、MXCV1、MXCV2.MWCV3,MXCV4等五菌株的生理生化特性研究表明:8406、MXCV1、MXCV2在YEM培养基中生长较快,代时在5小时以下;而MXCV_3;MXCV4生长较慢,代时分别为8.0和8.4小时.所有菌株在YEM培养基中产酸,对单糖、双糖、多糖等多种碳源的利用行为相似,在碳源利用时大多产酸,对抗菌素的抗性特征等都与一般快生型根瘤菌相似.菌株对有机氮源利用较好,而利用无机氮源生长较差.石蕊牛奶反应中均出现血清环、产酸或产碱,菌株在34℃以上高温均不生长,生长pH范围为6.0—8.5,菌株的耐盐能力不超过0.1mol/L NaCl.小冠花——根瘤菌共生效应表明,菌株MXCV_2的接种效果最显著,有作为优良菌株用于生产菌剂的前景.

STONES ON THE BIOLOGICAL CHARACTERISTICS OF RHIZOBLUB SP.(CoRONILLA Varia)

All the five root nodule bacterial strains, MXCVl, MXCV2, MXCV3, MXCV4, 8406, isolated from the nodules of Coronilla Varia can be identified as fast-growers. These strains can utilize a variety of carbon sources accompanied by acid-production, including monosaccharides, disaccharides and polysaccharides. Their resistance of antibiotics including kanamycia, tetracyclin and streptomycin accords with fast-growing standard. In litmus milk reaction, they show difference in acid- or alkali-production. No strain can tolerate high temperature and they cannot grow in the presence of 0.1 mol/L NaCl except MXCV 2 . But they all grow over a range fo pH 6-8.5. It is proposed that MXCV 2 may be the potenial candidate for inoculum.production on the basis of symbiotic characterization.