马尾松毛虫性外激素的触角电位(EAG)活性组分的分离、鉴定与合成

关于马尾松毛虫(Dendrolimus punctatus Walker)的性引诱现象及其性外激素柱层析分离的研究前已作过报道。实验结果表明,马尾松毛虫的性外激素提取物至少含有两种以上的触角电位(EAG)活性组分。可以设想,它的性外激素通讯系统和一般鳞翅目的相似,也是由多组分构成的。本文将报道我们结合使用气液色谱分离与EAG活性测定技术,确定色谱图中

ISOLATION, IDENTIFICATION AND SYNTHESIS OF EAG-ACTIVE COMPONENTS OF THE PINE-CATERPILLAR MOTH SEX PHEROMONE

For the removal of high boiling lipid contaminants and the enrichment of target components, crude extracts of the sex pheromone of female pine-caterpillar moth, Dendrolimus punctatus Walker, were first subjected to nitrogen sweep co-distillation. Furhter isolation were then, done through both OV-101 and CHDMS GC-eolumns, and 3 GC peaks from the preliminarily purified condensates of the co-distillation were found to be EAG active. GC-MS analyses indicated that 2 of the active peaks possessed the structures of dodecadienyl alcohol and its acetate separately. The positions and configurations of their double unsaturations were determined by ozonolysis and electroantennogram techniques. From the obtained analytical data, it is suggested that the structures of the 2 EAG active peaks might be Z5, E7-dodecadien-1-ol and its corresponding acetate. Four geometric isomers of each of these compounds were synthesized and purified by highpressure liquid chromatography. Field tests of their efficacy as male attractants are nowunder way.