恶性实体瘤患者自体CIK细胞的体外大量扩增后的免疫表型变化

目的:回顾性分析我院2009年1月至2010年5月行细胞因子诱导的杀伤细胞(CIK)治疗的59例实体瘤患者的自体CIK细胞体外扩增后免疫表型的变化,为恶性实体瘤患者开展CIK细胞治疗提供实验依据。方法:采集59例恶性实体瘤患者外周血单个核细胞,培养体系加入IFN-γ、CD3McAb及IL-2三种细胞因子体外诱导,培养10～14 d;用流式细胞仪分别检测CIK细胞培养前后的免疫表型,进行配对t检验。结果:CIK细胞培养后单个核细胞,CD3+细胞,CD3+CD4+细胞,CD3-CD56+细胞,CD3+CD56+细胞均较培养前增加,差异有统计学意义(P<0.05)。CD3+CD8+细胞,虽然较前增加,但差异无统计学意义(P>0.05),CIK细胞中CD3+,CD3+CD4+,CD3+CD8+,CD3-CD56+表型的细胞比率与培养前相比均有所下降,差异有统计学意义(P<0.05),但CD3+CD8+细胞比率与培养前差异无统计学意义(P>0.05)。而CIK细胞的纯度从培养前(9.90±8.96)%增至(46.55±19.25%),差异有统计学意义(P<0.05)。结论:恶性实体瘤患者自体CIK细胞体外扩增后,CIK细胞纯度显著增加。

Immunophenotype Changes of Auto-Cytokine Induced Killer Cells from Patients with Malignant Solid Tumor after Amplification in Vitro

Objective： To retrospectively analyze the changes of immunophenotyping of auto-cytokine induced killer cells from 59 patients with malignant solid tumor after amplification from January 2009 to May 2010,and to provide experimental evidence to CIK therapy for malignant solid tumor patients.Methods： Peripheral blood mononuclear cells（PBMC） of 59 patients with malignant solid tumor were collected.PBMC were cultivated for 10-14 days in vitro with traditional cultivating system added IFN-γ,CD3McAb and IL-2.Then,immunophenotype changes of CIK cells before and after culture were measured with flow cytometry（FCM）.The results then were conducted with matched t test.Results： After cultured,the PBMC,CD3 ＋ cells,CD3 ＋ CD4 ＋ cells,CD3-CD56 ＋ cells,CD3＋ CD56＋ cells increased significantly compared with that of before（P0.05）.CD3＋CD8＋cells showed slight but not significant increase（P0.05）.The percentage of CD3＋,CD3＋ CD4＋,CD3-CD56＋ phenotype cells declined with statistical significance（P0.05）,while the percentage of CD3＋CD8＋ cells had no significant difference（P0.05）.The purity of CIK cells after being cultured（46.55±19.25）% was significantly higher（9.90±8.96）%.Conclusion： The purity of CIK cells from patients with malignant solid tumor increased significantly after amplification in vitro.