| Archaeal acylamino acid releasing enzyme/lipase:Crystallization and preliminary crystallographic analysis in a new crystal form |
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| 作者姓名： | WANGGanggang GAORenjun |
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| 作者单位： | WANG Ganggang1，GAO Renjun2，YANG Haitao1，CAO Shugui2，FENG Yan2 & RAO Zihe1 1. MOE Laboratory of Protein Science & Laboratory of Structural Bio- logy，Department of Biological Science and Biotechnology，Tsinghua University，Beijing 100084，China; 2. Key Laboratory for Molecular Enzymology and Engineering of Min-istry of Education，Jilin University，Changchun 130023，China Correspondence should be addressed to Rao Zihe (e-mail: raozh@xtal. tsinghua.edu.cn) or Feng Yan (e-mail: yfeng@mail.jlu. edu.cn) |
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| 基金项目： | This work was supported by the 73 Project (Grant No. G1999075602). |
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| 摘 要： | A primitive orthorhombic crystal form of acylamino acid releasing enzyme‘lipase(APE1547)from hyperthermophilic archaeon Aeropyrum pernix strain K1 has been obtained at 291K.The diffraction pattern of the crystal extends to 0.27nm resolution at 100K using Cu Kαradiation.The crystal belongs to the space group P212121 with unit cell dimensions of b=6.399,b=10.439and c=16.953nm.The presence of two molecules per asymmetric unit gives a crystal volume per protein mass(Vm)of 0.0022nm^3 Da^-1 and a solvent content of 43% by volume.A full set of X-ray diffraction data were collected to 0.3nm from the native crystal.
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| 关 键 词： | 古细胞 脂肪酶 结晶形成 晶体结构分析 酰基肽水解酶 |
| 收稿时间： | 2002-08-29 |
Archaeal acylamino acid releasing enzyme/lipase:Crystallization and preliminary crystallographic analysis in a new crystal form |
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| Ganggang Wang, Renjun Gao, Haitao Yang, Shugui Cao, Yan Feng and Zihe Rao.Archaeal acylamino acid releasing enzyme/lipase:Crystallization and preliminary crystallographic analysis in a new crystal form[J].Chinese Science Bulletin,2003,48(2):154-156. |
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| Authors: | Ganggang Wang Renjun Gao Haitao Yang Shugui Cao Yan Feng and Zihe Rao |
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| Institution: | (1) MOE Laboratory of Protein Science & Laboratory of Structural Biology, Department of Biological Science and Biotechnology, Tsinghua University, 100084 Beijing, China;(2) Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, Jilin University, 130023 Changchun, China |
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| Abstract: | A primitive orthorhombic crystal form of acylamino acid releasing enzyme/lipase (APE1547) from hyperthermophilic archaeon Aeropyrum pernix strain K1 has been obtained at 291 K. The diffraction pattern of the crystal extends to 0.27 nm resolution at 100 K using Cu Ka radiation. The crystal belongs to the space group P212121 with unit cell dimensions of a = 6.399, b = 10.439 and c = 16.953 nm. The presence of two molecules per asymmetric unit gives a crys-tal volume per protein mass (Vm) of 0.0022 nm3 Da-1 and a solvent content of 43% by volume. A full set of X-ray dif-fraction data were collected to 0.3 nm from the native crys-tal. |
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| Keywords: | AARE Aeropyrum pernix orthorhombic crystal asymmetric unit |
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