鲨鱼肝蛋白粗提物对大鼠肝线粒体抗氧化功能的影响

摘以400mg／kg2次腹腔注射硫代乙酰胺（TAA）建立大鼠急性肝损伤模型，并在注射TAA前1h以80mg／kg2次腹腔注射鲨鱼肝蛋白粗提物进行预防，研究了鲨鱼肝蛋白粗提物对大鼠肝线粒体抗氧化功能的影响。注射TAA后，大鼠肝脏线粒体腺苷二磷酸（ADP）诱导的氧消耗、呼吸控制率（RCR）、磷／氧比（P／O）、氧化磷酸化率均低于对照组，而预防组线粒体ADP诱导的氧消耗、呼吸控制率、P／O和氧化磷酸化效率均高于模型组；TAA降低了线粒体中谷胱甘肽、谷胱甘肽还原酶、谷胱甘肽过氧化物酶的水平，而鲨鱼肝蛋白粗提物则使线粒体中谷胱甘肽过氧化物酶和超氧化物歧化酶的水平明显升高，说明鲨鱼肝蛋白粗提物能部分修复线粒体受损的呼吸功能，增强线粒体抗氧化能力。

Effects of Shark Protein Crude Extract Administration on Liver Mitochondrial Antioxidant Defenses in Rats

The effects of shark protein crude extract administration on liver mitochondrial antioxidant defenses were investigated in rats.Rats were given two consecutive intraperitoneal injections of(thioace-)tamide(TAA,400 mg/kg body weight) and shark protein extract(80 mg/kg body weight) 1 h before TAA treatment with a 24 h interval in model and therapy groups respectively.TAA administration(decreased) the ADP stimulated oxygen consumption,respiratory control ratio(RCR),the P/O value and oxidative phosphorylation ratio in isolated mitochondria,but shark protein extract could suppressed the(effect) of TAA.TAA administration decreased the levels of(glutathione,) glutathione peroxidase,(glutathione) reductase in mitochondria,and shark protein extract administration increased the levels of(glutathione) peroxidase and superoxide dismutase.These illustrated that shark protein extract could (partially) repair mitochondrial respiratory function impaired by TAA administration,and increase(mitochondrial)(antioxidant) defenses.