Purification and some properties of hemagglutinin from the Myxomycete,Physarum polycephalum |
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| Authors: | M Yokota K Nitta |
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| Institution: | (1) Department of Health Sciences, Asahikawa College of Hokkaido University of Education, Hokumon-cho 9, 070 Asahikawa, (Japan);(2) Cancer Research Institute, Tohoku College of Pharmaceutical Sciences, Komatsushima 4-4-1, Aoba-ku, 981 Sendai, (Japan) |
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| Abstract: | A new hemagglutinin was isolated from the plasmodium ofPhysarum polycephalum by salting out with ammonium sulphate followed by chromatography on DE-32, DEAE-Toyopearl and hydroxyapatite. This hemagglutinin, named physarumin, was purified 1000-fold over crude extracts. The molecular weight of physarumin was determined to be 22,000 by size exclusion chromatography on Bio-Gel P-60 and 8,700 by SDS-polyacrylamide gel electrophoresis. Physarumin agglutinated rabbit, guinea pig, horse and human erythrocytes. Physarumin-induced hemagglutination was inhibited by fetuin and  1 glycoprotein, but not by commercially available mono-and disaccharides. Hemagglutinating activity was blocked by EDTA, and was restored by adding Ca2+ but not by Mg2+. |
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| Keywords: | Myxomycete Physarum hemagglutinin Ca2+-dependent lectin |
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