黄芪丹参配伍提取物经VEGF、Ang1／Tie2通路对MI大鼠血管新生的病理影响

为观察黄芪、丹参配伍提取物对心肌梗死大鼠VEGF（vascular endothelial growth factor）、血管生成素Ang1及其受体酪氨酸激酶Tie2信号通路的表达影响。通过心肌梗死大鼠模型复制成功后分为假手术对照组、模型组、黄芪、丹参配伍提取物低、中、高剂量组，分别予以对应药物灌胃4周。观察大鼠一般生活状态，并分别采用HE染色和免疫组织化学染色测试大鼠心肌组织病理形态结构和VEGF、Ang1、Tie2的蛋白表达。结果表明：模型组心肌结构紊乱伴有炎性侵润，VEGF、Ang1和Tie2蛋白表达较假手术对照组有所增加（P＜0.05）；低、中、高剂量组随着药物浓度梯度增加，心肌结构逐渐规整，内皮细胞完整、新生血管增多，与模型组比较差异显著（P＜0.05或P＜0.01）。可见黄芪、丹参配伍提取物可以上调心肌梗死大鼠VEGF、Ang1和Tie2的表达，促进血管新生。

CompatibilitySofSAstragalusSandSSalviaSmiltiorrhizaSBge bySVEGF,SAng1/Tie2SPathwaySinSMISRats Pathological EffectsSofSAngiogenesis

In order to observe the effects of extracts of Astragalus and Salvia miltiorrhiza on the expression of VEGF (vascular endothelial growth factor), angiopoietin Ang1 and its receptor tyrosine kinase Tie2 signaling pathway in rats with myocardial infarction. METHODS: Rat models of myocardial infarction were successfully divided into sham-operated control group, model group, the extracts of Astragalus and Salvia miltiorrhiza in low, medium and high dose groups, and the corresponding drugs were administered for 4 weeks. The general living conditions of rats were observed, and the pathological structure of myocardium and the expressions of VEGF, Ang1 and Tie2 were measured by HE staining and immunohistochemical staining. RESULTS: The result showed that myocardial structural disorder was associated with inflammatory infiltration in the model group. The expression of VEGF, Ang1 and Tie2 protein was increased compared with the sham-operated control group (P<0.05). The low, medium and high dose groups increased with the concentration gradient of the drug. The structure was gradually regularized, the endothelial cells were intact, and the neovascularization was increased, which was significantly different from the model group (P<0.05 or P<0.01). CONCIUSION: It is concluded that the extracts of Astragalus and Salvia miltiorrhiza can up-regulate the expression of VEGF, Ang1 and Tie2 in rats with myocardial infarction and promote angiogenesis.