| CCK-8法和MTT法检测人前列腺癌PC3细胞活性的最佳条件比较研究 |
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| 引用本文： | 李宁,万文婷,金林红.CCK-8法和MTT法检测人前列腺癌PC3细胞活性的最佳条件比较研究[J].贵州大学学报(自然科学版),2009,26(3):18-20,24. |
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| 作者姓名： | 李宁 万文婷 金林红 |
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| 作者单位： | 贵州大学精细化工研究开发中心,教育部绿色农药与生物工程重点实验室,贵州,贵阳,550025 |
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| 摘 要： | 以人前列腺癌PC3细胞作为研究对象，探讨了CCK-8法和MTT法的最佳实验条件。CCK-8的最佳检测波长为450nm，最佳检测时间为加入CCK-8试剂后4h，适宜细胞数量范围为8×10^2-1×10^5个。在检测抗肿瘤药物阿霉素（ADM）对PC3细胞的增殖影响实验中，发现CCK-8法测得数据的线性相关性优于MTT法，并且数据偏差较MTT法小。实验结果表明CCK-8法较MTT法检测的灵敏度高，准确性好，是一种优于MTT法的检测细胞增殖活性的方法。
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| 关 键 词： | CCK-8 MTT 前列腺癌 细胞增殖 |
Optimal Experiment Conditions of Comparison between CCK-8 and MTT in Cell Proliferation Assays on PC3 Cell |
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| LI Ning,WAN Wen-ting,JIN Linhong.Optimal Experiment Conditions of Comparison between CCK-8 and MTT in Cell Proliferation Assays on PC3 Cell[J].Journal of Guizhou University(Natural Science),2009,26(3):18-20,24. |
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| Authors: | LI Ning WAN Wen-ting JIN Linhong |
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| Institution: | Research and Development Center of Fine Chemicals;Key Laboratory of Green Pesticide & Bioengineering;Ministry of Education;Guizhou University;Guiyang 550025;China |
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| Abstract: | The optimal experiment conditions of CCK-8 and MTT in PC3 cell proliferation assays were investigated. For CCK,8 assay, the best condition is ： 450 nm for detecting wave length, detected at 4 h after treatment, the number of PC3 cells is around 8 × 10^2 - 1 × 10^5. The antiproliferation action of ADM on PC3 were tested by CCK-8 and MTT. It was found that the result of CCK-8 method has better linear eorrelation fact, smaller deviation, higher sensitivity and accuracy. The conclusion is that CCK-8 method is superior to MTr method in cell proliferation assays. |
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| Keywords: | CCK-8 MTT |
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