| 过表达番茄叶绿体小分子热激蛋白提高植株的耐热性 |
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| 引用本文： | 杨传燕,王翠,张景霞,王佳颖,王丽,刘箭.过表达番茄叶绿体小分子热激蛋白提高植株的耐热性[J].山东师范大学学报(自然科学版),2008,23(4). |
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| 作者姓名： | 杨传燕 王翠 张景霞 王佳颖 王丽 刘箭 |
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| 作者单位： | 山东师范大学生命科学学院,250014,济南 |
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| 基金项目： | 国家自然科学基金资助项目(30270132) |
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| 摘 要： | 小分子热激蛋白是植物受到热胁迫后的主要表达产物之一，与植物细胞耐热有密切关系．我们将由CaMV35S启动子驱动的叶绿体小分子热激蛋白cDNA导入番茄，Northern及Western印迹分析表明，叶绿体小分子热激蛋白在转基因番茄中呈现组成型表达，通过测定转基因番茄和未转基因番茄的耐热性，发现转基因株系基础耐热性强于对照，说明叶绿体小分子热激蛋白的过量表达提高了植株的基础耐热性．
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| 关 键 词： | 热激蛋白 叶绿体 叶绿体小分子热激蛋白 耐热性 |
OVEREXPRESSION OF CHLOROPLAST- LOCALIZED SMALL MOLECULAR HEAT -SHOCK PROTEIN ENHANCES THE THERMOTOLERANCE IN PLANT |
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| Yang Chuanyan,Wang Cui,Zhang Jingxia,Wang Jiaying,Wang Li,Liu Jian.OVEREXPRESSION OF CHLOROPLAST- LOCALIZED SMALL MOLECULAR HEAT -SHOCK PROTEIN ENHANCES THE THERMOTOLERANCE IN PLANT[J].Journal of Shandong Normal University(Natural Science),2008,23(4). |
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| Authors: | Yang Chuanyan Wang Cui Zhang Jingxia Wang Jiaying Wang Li Liu Jian |
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| Abstract: | The small heat shock protein is usually the most heat responsive of the HSP,and is tightly related to the thermotolerance in organisms.A DNA construction,including tomato chloroplast-localized small molecular heat shock protein cDNA under the control of cauliflower mosaic virus 35S promoter,was introduced into the genome of tomato plants.Both Northern-blotting and Western-blotting analyses indicated that the constitutive expression of Le-CPsHSP in transgenic plants.Upon high temperature,the transgenic plant... |
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| Keywords: | HSPs chloroplast CPsHSP thermotolerance |
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