| 高效液相色谱法测定当归中阿魏酸的含量 |
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| 引用本文： | 陈超超,王艳,梁超.高效液相色谱法测定当归中阿魏酸的含量[J].成都大学学报(自然科学版),2008,27(4). |
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| 作者姓名： | 陈超超 王艳 梁超 |
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| 作者单位： | 成都中医药大学,药学院,四川,成都,610075;天津中医药大学,药学院,天津,300193 |
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| 摘 要： | 目的:建立一种高效液相色谱法测定当归中阿魏酸的含量.方法:采用高效液相色谱法,色谱柱为TIANHE C18(250 mm×4.6 mm,5μm),以甲醇-水-冰醋酸(40∶60∶0.5)为流动相,流速为1 mL/min,检测波长为320 nm.结果:阿魏酸在0.036~0.216μg范围内,线形关系良好(r=0.9999),平均回收率为101.4%(n=5),RSD为0.76%.结论:该方法测定阿魏酸含量简便、快捷、准确.
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| 关 键 词： | 高效液相色谱法 当归 阿魏酸 含量测定 |
Determination of Ferulic Acid in Radix Angelicae Sinensis by High Performance Liquid Chromatography Method |
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| CHEN Chaochao,WANG Yan,LIANG Chao.Determination of Ferulic Acid in Radix Angelicae Sinensis by High Performance Liquid Chromatography Method[J].Journal of Chengdu University (Natural Science),2008,27(4). |
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| Authors: | CHEN Chaochao WANG Yan LIANG Chao |
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| Institution: | CHEN Chaochao1,WANG Yan2,LIANG Chao2(1.School of medicine,Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China,2.School of medicine,Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China) |
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| Abstract: | Objective:A high performance liquid chromatographic (HPLC) method has been developed for determination of ferulic acid in radix angelica sinensis.Method:The ferulic acid was determined by HPLC.A 250 mm×4.6 mm,TIANHE C18 column with mobile phase MeOH∶H2O∶CH3COOH(40∶60∶0.5),at flow rate 1.00 mL·min-1 and detection at wavelength of 320 nm.Result:The linear range of ferulic acid was in the ranges of 0.036～0.216 ug,(r=0.9999),and the recovery was 101.4% with RSD 0.76%(n=5).Conclusion:HPLC was a simple,fast and accurate method for ferulic acid analysis. |
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| Keywords: | HPLC angelica sinensis ferulic acid quantitative determination |
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