辅酶Ⅰ诱导Cerasome聚集及乳酸脱氢酶对聚集的影响

用紫外可见分光光度计分别在500 nm和340 nm考察了由肽脂质—溴化N,N-二-十六烷基-Nα-6-三乙氧基硅基己酰基-L-甘氨酰胺（（EtO）3SiC3N＋C5Ala2C16）形成的阳离子脂质体——Cera-some,在还原型辅酶I（NADH）的作用下,以及在乳酸脱氢酶（LDH）和底物丙酮酸的存在下,其光密度（吸光度）随时间的变化。结果表明：随着NADH浓度的增加,Cerasome悬液的光密度（吸光度）明显增加,脂质体产生了聚集,但是聚集体不能被乳酸脱氢酶重新分散;而由溴化N,N-二-十六烷基-Nα-6-三甲胺基己酰基-L-丙氨酰胺（N＋C5Ala2C16）形成的阳离子脂质体聚集体,在乳酸脱氢酶的作用下,可以被重新分散。

On Aggregation of Cerasome Induced by Coenzyme I and Effects of Lactate Dehydrogenase on Aggregation

The over-time variation of the optical density（or absorbance） of cerasome,a cationic liposome prepared from a peptide lipid,N,N-dihexadecyl-Nα-[6-[（3-triethoxysilyl）-propyl dimethyl ammonio）hexanoyl] alaninamide bromide（（EtO）3SiC3N＋C5Gly2C16）,under the action of a reducing coenzyme I（NADH） and in the presence of lactate dehydrogenase（LDH） and the substrate,pyruvate,was studied using the ultraviolet-visible spectrophotometer at 500 nm and 340 nm respectively.The result showed that： the aggregation of the liposome occurred as the optical density（or absorbance） of the cerasome suspension increased obviously along with the concentration of NADH,but it couldn＇t be redispersed by LDH;nonetheless,the aggregation of a cationic liposome prepared from N,N-dihexadecyl-Nα-[6-（trimethyl ammonio）hexanoyl]alaninamide bromide（N＋C5Ala2C16） could be redispersed by LDH.