| 肌苷高产菌TSXB29的转化育种及其发酵条件的研究 |
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| 引用本文： | 陈宁,孙振环,刘永生,张克旭.肌苷高产菌TSXB29的转化育种及其发酵条件的研究[J].天津科技大学学报,1995(1). |
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| 作者姓名： | 陈宁 孙振环 刘永生 张克旭 |
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| 作者单位： | 天津轻工业学院食品工程系 |
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| 摘 要： | 以枯草杆菌（Ｂａｃｉｌｌｕｓｓｕｂｔｉｌｉｓ）ＴＸ２（遗传标记Ｘａｎ~－）为供体，以枯草杆菌ＴＳＡ１９（遗传标记Ａｄｅ~－＋Ｈｉｓ~－＋８－ＡＧ~ｒ＋６－ＭＰ~ｒ＋ＳＧ~ｒ）为受体，利用转化法成功地获得肌苷产量高、遗传稳定性好的目的转化子ＴＳＸＢ２９。采用均匀设计理论，利用微机对实验数据进行统计处理，找出了该转化子的最佳摇瓶发酵条件，在该条件下，平均产苷为２７．８５ｇ／Ｌ。经连续摇瓶和斜面传代，发现该转化子是稳定的，具有一定应用价值。
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| 关 键 词： | 转化，均匀设计，肌苷，发酵 |
STUDIES ON THE TRANSFORMATION BREEDING OF HIGH INOSION PRODUCING STRAIN TSXB29 AND ITS FERMENTABLE CONDITIONS |
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| Chen Ning,Sun Zhenhuan,Liu Yongsheng,Zhang Kexu.STUDIES ON THE TRANSFORMATION BREEDING OF HIGH INOSION PRODUCING STRAIN TSXB29 AND ITS FERMENTABLE CONDITIONS[J].Journal of Tianjin University of Science & Technology,1995(1). |
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| Authors: | Chen Ning Sun Zhenhuan Liu Yongsheng Zhang Kexu |
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| Institution: | Chen Ning;Sun Zhenhuan;Liu Yongsheng;Zhang Kexu |
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| Abstract: | Bacillus subtilis TX2(Xan~-)being used as the donor,Bacillus subtilis TSA19(Ade~- His~- 8-AG~r 6-MP~r SG~r) as the recipient, with the method of transformation, a stable higher inosine-productivity transformant TSXB29 was obtained successfully. Using the theory of Uniform Design, the experimented data were analysed with the computer, then the optimum flaskshaking fermentable conditions were determined. under the optimum conditions, the transformant accumulates an average of 27.85g/l of inosine. After several generations in the shaking flask and several generations on the slope,the transformant was found stable,so it has the value of application. |
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| Keywords: | Transformation Uniform design Inosine Fermentation |
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