| Multiple transgenes Populus xeuramericana ＇Guariento＇ plants obtained by biolistic bombardment |
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| 作者姓名： | Wang JianGe Su XiaoHua Ji LiLi Zhang BingYu Hu ZanMin Huang RongFeng Tian YingChuan |
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| 作者单位： | [1]Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China [2]College of Landscape and art, Jiangxi Agricultural University, Nanchang 330045, China [3]Institute of Genetics and developmental biology, Chinese Academy of Sciences, Beijing 100101, China [4]Biotechnology Research Institute, Chinese Academy of Agricultural Science, Beijing 100081, China [5]Microbiology Institute, Chinese Academy of Sciences, Beijing 100080, China |
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| 基金项目： | The authors would like to thank Dr. Hu Jun for Southern blotting. |
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| 摘 要： | A JERF36 regulation gene, a selection marker gene （NPT-Ⅱ）, and the foreign genes levansucrase （SacB）, Vitreoscilla hemoglobin （vgb）, and Binary coleopterus insect resistance （BtCry3A＋OC-I） were co-transferred into Populus xeuramericana ＇Guariento＇ using biolistic bombardment; 25 kanamycin resistant plants were obtained, The results of PCR and Southern hybridization showed that the foreign genes had been integrated into the genome of P, xeuramericana ＇Guariento＇ and 5 genes were all transferred into 7 poplar plants, The results of a BtCry3A ELISA experiment indicated that the BtCry3A gene was expressed in the 7 transgenic poplar plants, and these plants grew well on coastal saline land,
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| 关 键 词： | 森林 生态系统 全球变暖 二氧化碳 植被 |
| 收稿时间： | 8 June 2006 |
| 修稿时间： | 2006-06-082006-11-02 |
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