磁性纳米粒子的制备及其细胞分离方面的应用

介绍了一种始终在溶液中制备Fe3O4磁性纳米粒子的化学共沉淀，并对制得的粒子进行表面修饰的方法．通过IR，XRD和AFM等测试仪器对样品进行了表征．结果表明：采用化学共沉淀的方法制备出的磁性纳米粒子具有单分散性，且粒径比较均一，约在30nm．由于制备过程均在液体中进行，故制备周期短，污染小．同时利用自制的免疫磁性Fe3O4纳米粒子能较好地分离脐血中的CD133细胞，细胞体外扩增明显．

Preparation of magnetic nano-particles and their application in cell separation

Ferriferous oxide nano-particles were prepared using chemical co-precipitation method and then the particles were modified by chemical reagent and the whole process was carried out in a solution. Infrared spectra, X - ray diffraction and atomic force microscope were applied to characterize the magnetic nano-particles. The results indicate that the well dispersed, non-polluted and bio-compatible nano-particles were obtained with their average diameter of around 30 nm. CD133 cells were well separated using this kind of magnetic nano-particles and a good increment was obtained.