| 22种丝状异型胞固氮蓝细菌的16S rDNA的 PCR-RFLP 分析 |
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| 引用本文： | 陈坚,吴忠兴,郑伟文.22种丝状异型胞固氮蓝细菌的16S rDNA的 PCR-RFLP 分析[J].厦门大学学报(自然科学版),2002,41(5):664-668. |
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| 作者姓名： | 陈坚 吴忠兴 郑伟文 |
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| 作者单位： | 福建省农科院生物技术中心,福建,福州,350003 |
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| 基金项目： | 福建省中 瑞国际合作项目 (99 Z 3) |
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| 摘 要： | 用1对引物CYA106F及781R（a）从23种蓝细菌的DNA中扩增出一条600bp的16SrDNA－PCR片段，对该片段进行的限制性酶切长度多态性（RFLP）分析表明：可将其中21种自生或共生的丝状异型胞固氮蓝细菌的样品分为2个类群组，基本上对应于形态分类上的Anabaena及Nostoc属，但有1种被认为是Anabaena的蓝细菌以及作为对照的1种聚球藻(Synechococcus)蓝细菌样品被明显区分出来，并发现Anabaena属的蓝细菌也可同被子植物根乃拉草（Gunnera）形成共生固氮作用。
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| 关 键 词： | 丝状异型胞 固氮蓝细菌 16SrDNA PCR－RFLP分析 蓝藻 念珠藻目 形态分类 聚球藻 |
| 文章编号： | 0438-0479(2002)05-0664-05 |
| 修稿时间： | 2002年5月20日 |
Analysis of 22 Strains of Nitrogen-fixing Filamentous Cyanobacteria with Heterocyst by Using PCR-RFLP of 16S rDNA |
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| CHEN Jian,WU Zhong-xing,ZHENG Wei-wen.Analysis of 22 Strains of Nitrogen-fixing Filamentous Cyanobacteria with Heterocyst by Using PCR-RFLP of 16S rDNA[J].Journal of Xiamen University(Natural Science),2002,41(5):664-668. |
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| Authors: | CHEN Jian WU Zhong-xing ZHENG Wei-wen |
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| Abstract: | A 16S rDNA-PCR fragment at about 600 bp was amplified from 23 cyanobacterial accessions by using primer pair CYA106F and 781R(a). The RFLP analysis of this fragment indicated that of these tested accessions, 21 strains of nitrogen-fixing filamentous cyanobacteria with heterocyst were separated into two clusters basically corresponding to Anabeana and Nostoc genus on the basis of morphological classification. but 1 strain identified as Anabeana and 1 strain of Synechococcus as check showed a greatly difference from the two clusters. The result also revealed that the Anabeana strain possibly formed symbiosis with angiosperm Gunnera for nitrogen fixation. |
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| Keywords: | cyanobacteria PCR-RFLP 16S rDNA symbiosis |
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