| 电针镇痛对小鼠脑细胞游离Ca2+浓度的影响 |
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| 引用本文： | 章明美,谢吉民,陈敏,张燕.电针镇痛对小鼠脑细胞游离Ca2+浓度的影响[J].江苏大学学报(自然科学版),2005,26(2):182-184. |
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| 作者姓名： | 章明美 谢吉民 陈敏 张燕 |
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| 作者单位： | 江苏大学化学化工学院,江苏,镇江,212013 |
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| 基金项目： | 江苏省自然科学基金资助项目(BK2001150) |
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| 摘 要： | 实验探讨电针镇痛及钙通道阻断剂盐酸异博定(verapamil)、硝苯吡啶(nifedipine)对小鼠海马细胞及突触体游离Ca^2 浓度的影响．采用荧光染料Fura-2／AM测定在电针镇痛期间，小鼠海马细胞及突触体内游离钙浓度(Ca^2 ]i)的改变．结果表明电针镇痛时，海马细胞内Ca^2 ]．升高，而突触体Ca^2 ]i降低，向分离出的突触体培养液内加入钙通道阻断剂盐酸异博定、硝苯吡啶，突触体Ca^2 ]．明显降低．提示电针的镇痛效应可能与海马神经细胞膜内、外钙离子浓度的变化有关．
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| 关 键 词： | 电针 海马细胞 突触体 游离钙 |
| 文章编号： | 1671-7775(2005)02-0182-03 |
| 修稿时间： | 2004年8月2日 |
Effects of electro-acupuncture in cytosolic free calcium concentrations on mice brain cells |
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| ZHANG Ming-mei,XIE Ji-min,CHEN Min,ZHANG Yan.Effects of electro-acupuncture in cytosolic free calcium concentrations on mice brain cells[J].Journal of Jiangsu University:Natural Science Edition,2005,26(2):182-184. |
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| Authors: | ZHANG Ming-mei XIE Ji-min CHEN Min ZHANG Yan |
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| Abstract: | The effects of electro-acupuncture and verapamil, nifedipine, and the calcium channel inhibitor on cytosolic free calcium concentrations in hippocampal and intrasynaptosomal of mice were investigated. The concentrations of cytosolic free calcium (Ca~(2+)]_i) in hippocampal and intrasynaptosomal of mice were determined by using the fluorescent calcium indicator Fura-2/AM. During the electro-acupuncture anelgesic period, the Ca~(2+)]_i in hippocampal cells was increased, but the Ca~(2+)]_i of intrasynaptosomal was decreased. The concentrations of cytoplasmic free calcium were significantly decreased if verapamil or nifedipine were added into the Abstracted intrasynaptosomal solution. It is indicated that the acupuncture effect maybe connected with the changes of Ca~(2+)]_i Hippocampal. |
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| Keywords: | electro-acupuncture Hippocampal intrasynaptosomal cytosolic Ca~(2+) |
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